胚胎大鼠神经干细胞的分离培养及其鉴定

郑敏; 王健; 王亚平; 裴雪涛

doi:10.13406/j.cnki.cyxb.2004.04.008

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胚胎大鼠神经干细胞的分离培养及其鉴定

更新时间：2024-01-17

- 胚胎大鼠神经干细胞的分离培养及其鉴定
- Isolation,expansion and identification of fetal rat neural stem cell
- 重庆医科大学学报 2004年第4期页码：433-436
- 作者机构：
  
  1. 重庆医科大学基础医学院组胚教研室
  2. 重庆医科大学第二临床学院神经内科
  3. 军事医学科学院干细胞研究室,重庆,400016
  4. ,北京,100085
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2004.04.008
  中图分类号： R329.2
- 纸质出版日期：2004，
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[1]郑敏,王健,王亚平,裴雪涛.胚胎大鼠神经干细胞的分离培养及其鉴定[J].重庆医科大学学报,2004(04):433-436.

ZHENG Min, et al. Isolation,expansion and identification of fetal rat neural stem cell[J]. Journal of Chongqing Medical University, 2004,(4):433-436.
[1]郑敏,王健,王亚平,裴雪涛.胚胎大鼠神经干细胞的分离培养及其鉴定[J].重庆医科大学学报,2004(04):433-436. DOI： 10.13406/j.cnki.cyxb.2004.04.008.

ZHENG Min, et al. Isolation,expansion and identification of fetal rat neural stem cell[J]. Journal of Chongqing Medical University, 2004,(4):433-436. DOI： 10.13406/j.cnki.cyxb.2004.04.008.

摘要

目的 :通过培养了解神经干细胞（neuralstemcell

NSC）生物学特性

为深入研究其体内外分化奠定基础。方法 :分离培养大鼠胎脑NSC。通过单细胞克隆实验检测其自我更新能力、增殖能力和生物学特性 ;免疫细胞化学检测NSC特征性标志nestin表达和分化能力 ;BrdU掺入实验检测细胞增殖状态 ;并比较EGF、bFGF对鼠NSC增殖刺激作用。结果 :体外传代培养获得的NSC具有多向分化能力、很强的自我更新能力、表达nestin ;NSC扩增迅速 ;传代培养的NSC性能稳定 ;联合运用EGF、bFGF对NSC增殖作用较单用强。结论 :成功培养扩增了大鼠NSC ;EGF、FGF联合作用能促进大鼠NSC增殖

Abstract

Objective:To culture neural stem cell (NSC) in vitro

observe its biological property and provide basis for further study on differentiation.Methods:NSC of rat fetus were isolated and cultured.Single-cell clone culture was used to confirm the character of self-renewing.Ability of multi-directional differentiation and specific antigen

nestin

were detected by immunocytochemistry (ICC).BrdU was used to show cell stage in division.Effect of combination of EGF

bFGF on proliferation of rat NSC were evaluated.Results:The cultured NSC showed multipotential of differentiation into neurons and glial cells

as well as the ability of self-renewing.Cells expressed nestin.The population of the NSC expanded quickly and the total number of the cell increased 2.45×10~4 folds after 15 times’ passage.The property of NSC was stable after long culture and preservation.Combination of EGF and bFGF stimulated cell proliferation than used singly.Conclusion:The cultured NSC express nestin

have the ability of self-renewing and multi-directional differentiation.Combination of EGF and bFGF could promote the proliferation of rat NSC.

关键词

神经干细胞自我更新多向分化扩增

Keywords

neural stem cellSelf-renewingMultipotential proliferationDifferentiation

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