CHEN Daorong, et al. Cloning and sequence analyzing of the recombinant plasmid affecting gene DNMT1 translation by RNA interfering[J]. Journal of Chongqing Medical University, 2004,(3):269-272.
CHEN Daorong, et al. Cloning and sequence analyzing of the recombinant plasmid affecting gene DNMT1 translation by RNA interfering[J]. Journal of Chongqing Medical University, 2004,(3):269-272. DOI: 10.13406/j.cnki.cyxb.2004.03.004.
Objective:To clone the recombinant plasmid affecting gene DNMT1 translation by RNA interfering and analyze the nucleic acid sequence of the recombinant for further searching new gene therapy method of tumor.Methods:Two DNA sequences containing small hairpin structure were designed and synthesized.The complement form was obtained by annealing and inserted into vector pTZU6+1
and the recombinant plasmid was transformed into Jm109 strain.Finally the plasmid identified by restriction enzyme was used for sequence analysis.Results:The recombinant was cloned and the aim sequence was obtained.Conclusion:Successful cloning of the recombinant helps to search new gene therapy method of tumor.