Objective:To provide a correlation evidence in laboratory for instruction breast-feeding

the investigation of positive rates with serum hepatitis B virus markers(HBVM) in parturients and their infants of breast-feeding to HBVM antigen positive mothers.Methods:The serum HBVM of pregnant women and their infants(12～18th month)were determined by ELISA and HBV-DNA in positive serum and colostrum were determined by fluorescence quantitative PCR

and whole process of immune-protection for pregnant women and their infants.The relation between HBVM in the infant serum and the feeding way was analysed.Results:In 67 case parturient of serum HBVM antigen positive

the positive rate of HBV-DNA was 84%

26% in serum and colostrum with HBeAg positive and HBeAg negative mothers;and there was a positive relationship between the serum and colostrum.The positive rate of HBeAg of serum mothers were 41% and 13% in the artificial feeding group and breast feeding group

there was significant difference between them( P <0.01).After 12～18 months

the infants positive rate of serum anti-HBs was 84% and 87% in the artificial feeding group and breast feeding group( P >0.05).Conclusion:As determinations of HBV-DNA were negative in colostrum

the breast-feeding was safe to HBVM antigen positive mothers by active and passive immunization with pregnant women and their infants.