急性肺损伤大鼠肺表面活性物质及肺表面活性物质蛋白的研究

许峰; 何勇; 匡凤梧; 卢仲毅; 王兴勇

doi:10.13406/j.cnki.cyxb.2004.02.004

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急性肺损伤大鼠肺表面活性物质及肺表面活性物质蛋白的研究

更新时间：2024-01-17

- 急性肺损伤大鼠肺表面活性物质及肺表面活性物质蛋白的研究
- Study of pulmonary surfactant and surfactant protein in rats with LPS-induced acute lung injury
- 重庆医科大学学报 2004年第2期页码：131-133
- 作者机构：
  
  1. 重庆医科大学儿科学院PICU
  2. 重庆医科大学儿科学院PICU,重庆,400014
- 作者简介：
- 基金信息：
  
  重庆市卫生局回国人员科研基金资助项目（基金号:0 0 60 0 2 ）
- DOI：10.13406/j.cnki.cyxb.2004.02.004
  中图分类号： R563.8
- 纸质出版日期：2004，
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[1]许峰,何勇,匡凤梧,卢仲毅,王兴勇.急性肺损伤大鼠肺表面活性物质及肺表面活性物质蛋白的研究[J].重庆医科大学学报,2004(02):131-133+152.

XU Feng, et al. Study of pulmonary surfactant and surfactant protein in rats with LPS-induced acute lung injury[J]. Journal of Chongqing Medical University, 2004,(2):131-133.
[1]许峰,何勇,匡凤梧,卢仲毅,王兴勇.急性肺损伤大鼠肺表面活性物质及肺表面活性物质蛋白的研究[J].重庆医科大学学报,2004(02):131-133+152. DOI： 10.13406/j.cnki.cyxb.2004.02.004.

XU Feng, et al. Study of pulmonary surfactant and surfactant protein in rats with LPS-induced acute lung injury[J]. Journal of Chongqing Medical University, 2004,(2):131-133. DOI： 10.13406/j.cnki.cyxb.2004.02.004.

摘要

目的 :探讨肺表面活性物质（PS）及肺表面活性物质蛋白（SP）在内毒素（LPS）性急性肺损伤（ALI）中的变化趋势。方法 :成年Wistar大鼠随机分为对照组（NS组）和肺损伤组（ALI组）

ALI组采用LPS诱导建立ALI模型。用定磷法和高效液相色谱法动态监测了NS组和ALI组第 1、3、5、7h大鼠支气管肺泡灌洗液（BALF）中的总磷脂（TPL）和磷脂各组分

采用逆转录聚合酶链反应（RT -PCR）的方法检测肺组织SP -A、SP -BmRNA的表达情况

此外对肺损伤的病理学改变也进行了对比分析。结果 :与NS组比较

ALI组有明显水肿、出血和炎症。BALF中TPL在 1、3、5、7h明显低于NS组（P <0 .0 5 ）

卵磷酯胆碱（PC）在 3、5、7h明显低于NS组（P <0 .0 5 ） ;而溶血卵磷脂（LPC）在 1、3、5、7h明显高NS组（P <0 .0 5 ）。肺组织SP -A、SP -BmRNA表达在 3、5、7h明显低于NS组（P <0 .0 5 ）。结论 :ALI存在PS的代谢改变

以及SP -A、SP -BmRNA的表达降低

PS含量的降低和组分的改变是导致难以纠正的低氧血症重要原因之一。

Abstract

Objective:To study the alteration trend of pulmonary surfactant(PS) and surfactant-associated protein(SP) in rats with LPS-induced acute lung injury(ALI).Methods:Fifty-six adult Wistar rats were randomly divided into normal saline(NS) group and ALI group.The levels of mRNA of the SP-A

SP-B were measured by reverse transcription polymerase chain reaction(RT-PCR) during iv LPS at 1

7h.The content and component of PS in the bronchoalveolar lavage fluid (BALF) were measured by high performance liquid chromatography (HPLC).In addition

studies on lung dry/wet weight ratio

the protein content of BALF

alveolar oxygen partial pressure (PaO 2)and histological changes were performed.Results:Compared with the NS group

ALI group developed severe lung damage.Edema

hemorrharge and inflammatory changes were found.Total phospholipids (TPL) of BALF at 1

7h were lower than those in the NS group( P <0.05);phosphatidylcholine(PC) at 3

7h were lower than those in the NS group( P <0.05);while lysophosphatidylcholine (LPC) at 1

7h were higher than thosein the NS group(P <0 .0 5 ) .Theexpression ofSP -A

SP -Bm RNAat 3

7h were less than thosein the NSgroup(P <0 .0 5 ) .Conclusion :The changed metabolism of PSex ists in the pathogenesisn of ALI

which is mainly de monstratedas that the decreasein TPL

will de crease expression of SP -A

SP -Bm RNA .Decreasein content and changein components of PS may play an important role in severe hypoxemia in ALI .

关键词

急性肺损伤肺表面活性物质大鼠

Keywords

acute lung injuryPulmonary surfactantrat

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