Objective:To investigate the effect of basic fibroblast growth factor (bFGF) on corneal cell viability in mid-term preserved medium.Methods:prepared the medium which contained minimum essential medium (MEM)

dextran (40000)

HEPES and so on.New Zealand′s white rabbits′ corneas were preserved in the medium and the medium with bFGF(10ng·ml -1 ) at 30～34℃ for 16～18 hours

and then transmited to 4℃to be storaged.At 3rd

5th

7th day after preservation

the viability of corneal endothelial cells (CECs) were assessed by trypan blue-alizarin red stain;the ultrastructure of comea was observed by electron microscope.Corneal cell proliferation was monitored by PCNA immunohistochemistry.Results:The corneal edema and folds in the medium with bFGF are slight.The cornea transparency

cell shape and the cell viability in bFGF group are better than that in matched control group(P<0.05 ).PCNA-positive cells in corneal epithelial

stromal and endothelial layers in experimental groups were significantly more than that in matched control group(P<0.05) .Conclusion:The results indicated that bFGF play a significant role in stimulating cell proliferation and maintaining the structure and viability.It can be administered to corneal mid-term preserved medium.