Objective:To study the relationship between cyclin dependent kinase 4 (CDK4)

PTEN protein expression and carcinogenisis

progression and recurrence of endometrial carcinoma.Methods:A specific and sensitive immunohistochemical S-P assay was used to detect the expression of PTEN and CDK4 in paraffin-embedded sections of 50 cases of endometrial carcinoma

12 atypical hyperplasia of endometrium and 10 normal endometrium.Results:1.The positive immunostainning rates of PTEN in endometrial carcinoma

atypical hyperplasia of endometrium and normal endometrium were 56.00%

91.00%

100.00%

respectively;and the difference was statistically significant between carcinoma and normal endometrium or atypical hyperplasia of endometrium(P<0.05).The expression of PTEN correlated with muscle invasion(P<0.05)

but not with histological grade or recurrence.2.The positive rate of CDK4 nuclear expression in carcinoma and in atypical hyperplasia of endometrium was significantly higher than that in normal endometrium(P<0.05).The nuclear positive expression and overexpression rates of CDK4 were significantly higher in stage Ⅱ～Ⅲ

moderately poorly differentiated(G 2-G 3)carcinoma

>1/2 muscle invasion cancer and in recurrence cases.Conclusion:The results suggest that the aberrant expressions of PTEN and CDK4 are closely related to the occurrence

development and recurrence of endometrial carcinoma.