YANG Zhu, et al. Experimental study on embryo culturing medium of malignant tumor growth fluid[J]. Journal of Chongqing Medical University, 2003,(5):564-567.
YANG Zhu, et al. Experimental study on embryo culturing medium of malignant tumor growth fluid[J]. Journal of Chongqing Medical University, 2003,(5):564-567. DOI: 10.13406/j.cnki.cyxb.2003.05.003.
Objective:To find a good culture medium for embryo development in-vitro.Methods:D4 mouse embryo was cultured with carcinoma of kidney GRC-1 growth fluid and FD+20% fetal calf serum respectively in-vitro and the rate of zona-hatching
attachment and trophoblast outgrowth were compared respectively.Results:The rate of zona-hatching
attachment and trophoblast outgrowth with GRC-1 were 89.68%
86.51 %和84.92%
respectively.These were significantly higher than those of the control group ( P <0.01 ).Conclusion:GRC-1 growth fluid is a kind of good culture medium for growth and development of mouse embryo in-vitro.