Objective:To study the biological functions of bone marrow macrophage (BMM Φ) and to establish the methodology of separation

purification

cultivation and identification of BMM Φ.Methods:Using the techniques of anchorage-dependent culture of separated rat bone marrow cell (rBMC) in DMEM culture media (contain 20% horse serum

20% (v/v)L 929 conditioned media) in vitro

a lot of purified anchor cells were obtained

and these cells were identified with specifically biological marker of macrophage

such as 1

morphologicalobservation:invert phase contrast microscopy

light and electron microscopy;2

enzyme cytochemistry:acid phosphatase (ACP)

α-acetic acid naphthol esterase (α-ANE);3

phagocytic experiment:phagocytosis of chicken erythrocytes and prepared Chinese ink;4

immunocytochemistry: surface specific antigen of macrophage ( CD 68 stain).Results:The cells were purified having functional satisfactory macrophage according to morphological observation

enzyme cytochemistry

phagocytic experiment and immunocytochemistry.Conclusion:This is a simple and easy method for separation

purification

cultivation and identification of rat marrow macrophage.