阈下电刺激促大鼠缺血心肌毛细血管新生的研究

佘强; 陈运贞

doi:10.13406/j.cnki.cyxb.2003.03.012

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阈下电刺激促大鼠缺血心肌毛细血管新生的研究

更新时间：2024-01-17

- 阈下电刺激促大鼠缺血心肌毛细血管新生的研究
- The study of angiogenesis induced by electrical stimulation below contraction threshold,in ischemic myocardium
- 重庆医科大学学报 2003年第3期页码：296-299
- 作者机构：
  
  1. 重庆医科大学第二临床学院心内科
  2. 重庆医科大学临床学院心内科,重庆,400010
  3. ,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.03.012
  中图分类号： R541
- 纸质出版日期：2003，
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[1]佘强,陈运贞.阈下电刺激促大鼠缺血心肌毛细血管新生的研究[J].重庆医科大学学报,2003(03):296-299.

SHE Qiang, et al. The study of angiogenesis induced by electrical stimulation below contraction threshold,in ischemic myocardium[J]. Journal of Chongqing Medical University, 2003,(3):296-299.
[1]佘强,陈运贞.阈下电刺激促大鼠缺血心肌毛细血管新生的研究[J].重庆医科大学学报,2003(03):296-299. DOI： 10.13406/j.cnki.cyxb.2003.03.012.

SHE Qiang, et al. The study of angiogenesis induced by electrical stimulation below contraction threshold,in ischemic myocardium[J]. Journal of Chongqing Medical University, 2003,(3):296-299. DOI： 10.13406/j.cnki.cyxb.2003.03.012.

摘要

目的 :探讨不同频率的阈下电刺激促缺血心肌毛细血管新生的作用及其发生机制。方法 :给心肌梗死大鼠发放不同频率的阈下电刺激

观察缺血心肌中毛细血管新生情况

以及血管内皮生长因子（VEGF）的表达、一氧化氮合酶（NOS）活性的变化。结果 :（1） 2 5Hz频率的阈下电刺激组

大鼠缺血心肌中毛细血管密度、VEGFmRNA及蛋白质表达、NOS活性均比其它电刺激组及对照组增加。（2 ） 2 5Hz频率阈下电刺激基础上加用VEGF中和抗体干预后

大鼠缺血心肌毛细血管密度明显减少

NOS活性降低。（3） 2 5Hz频率阈下电刺激基础上加用NOS抑制剂L -NAME干预后

大鼠缺血心肌中VEGFmRNA及蛋白质表达没有改变

但毛细血管密度显著减少。结论 :2 5Hz频率的阈下电刺激可以促进缺血心肌中VEGF表达的上调

导致毛细血管新生 ;NO可能介导VEGF促血管内皮细胞增殖作用。

Abstract

Objective:To study the ischemic myocardial angiogenesis induced by electrical stimulation below contraction threshold.Methods:24 infarcted rats were given electrical stimulation below contraction threshold for 5 days with 10Hz

15Hz

25Hz

50Hz stimulus frequencies.12 other infarcted rats were electrically stimulated with effective stimulus frequency which induced myoardial angiogenesis and treated with VEGF neutralizing antiboby

L-NAME for 5 days.Capillary density(CD)

activity of NOS

expression of VEGF mRNA and protein

were detected.Results:CD

activity of NOS

expression of VEGFmRNA and protein in ischemic myocardium were significantly augmented in the 25Hz group compared with the other groups.CD and activity of NOS in ischemic myocardium significantly decreased in 25Hz+VEGF neutralizing antibody group compared with that of the 25Hz group.CD in ischemic myocardium significantly decreased in the 25Hz+L-NAME group compared with that of the 25Hz group

but expression of VEGF mRNA and protein were in the absence of changes the 2 groups.Conclusion:Myocardial angiogenesis induced by electrical stimulation below contraction threshold at 25Hz frequeney is dependent on the expression of VEGF.Nitric oxide can mediate mitogenic effect of VEGF on capillary endothelial cells.

关键词

血管新生电刺激血管内皮细胞生长因子

Keywords

angiogenesiselectrical stimulationvascular endothelial growth factor

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