荷瘤小鼠多药耐药（MDR1）基因转染后P-gp体内表达的研究

郭春宝; 金先庆; 康权; 安淑华; 王珊

doi:10.13406/j.cnki.cyxb.2003.03.008

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荷瘤小鼠多药耐药（MDR1）基因转染后P-gp体内表达的研究

更新时间：2024-01-17

- 荷瘤小鼠多药耐药（MDR1）基因转染后P-gp体内表达的研究
- The expression of P-gp during the MDR1 gene transferring into the hematopoietic cells of the Balb/c mouse bone marrow
- 重庆医科大学学报 2003年第3期页码：279-283
- 作者机构：
  
  1. 重庆医科大学儿科学院普外科
  2. 重庆医科大学儿科学院普外科,重庆,400014
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目（No .3 0 0 70 781）
- DOI：10.13406/j.cnki.cyxb.2003.03.008
  中图分类号： R73-36
- 纸质出版日期：2003，
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[1]郭春宝,金先庆,康权,安淑华,王珊.荷瘤小鼠多药耐药(MDR1)基因转染后P-gp体内表达的研究[J].重庆医科大学学报,2003(03):279-283.

GUO Chunbao, et al. The expression of P-gp during the MDR1 gene transferring into the hematopoietic cells of the Balb/c mouse bone marrow[J]. Journal of Chongqing Medical University, 2003,(3):279-283.
[1]郭春宝,金先庆,康权,安淑华,王珊.荷瘤小鼠多药耐药(MDR1)基因转染后P-gp体内表达的研究[J].重庆医科大学学报,2003(03):279-283. DOI： 10.13406/j.cnki.cyxb.2003.03.008.

GUO Chunbao, et al. The expression of P-gp during the MDR1 gene transferring into the hematopoietic cells of the Balb/c mouse bone marrow[J]. Journal of Chongqing Medical University, 2003,(3):279-283. DOI： 10.13406/j.cnki.cyxb.2003.03.008.

摘要

目的 :探讨恶性肿瘤MDR1基因治疗过程中

MDR1基因在体内的表达。方法 :骨髓造血细胞转染MDR1基因后

程序性移植入预先照射亚致死剂量60 Co -γ射线的荷瘤小鼠

取骨髓造血细胞、肿瘤实体做P -糖蛋白免疫组化并做RT -PCR以观察MDRI基因的表达情况。结果 :各剂量组MDRI基因转染后随时间骨髓造血细胞P -糖蛋白表达逐渐增加

各剂量组之间无明显差异。P -糖蛋白表达化疗剂量较大组明显高于化疗剂量较小组。结论 :MDRI基因转染在骨髓造血细胞有较高表达

对肿瘤实体、肝脏、肾脏P -糖蛋白表达无影响。

Abstract

Objective:The study was to improve the protection effect of the bone marrow during the chemotherapy of earlier hepatocarcinoma Balb/C mice

to observe the expression of P-gp in tumor tissues and important organs and to examine the influence of MDR1 gene on Balb/C mouse. Methods:MDR1 gene was transferred into hematopoietic cells of murine bone marrow by the mediation of retrovirus vecter and transplanted into the Balb/c mouse with hepatocarcinoma exposure with 60 Co-γ ray in advance.The function and the expression of MDR1 gene in hematopoietic cells

and tumor tissue was detected by RT-PCR method and IC method in vitro.Results:There was no P-gp expression in the two control groups

but that of the different dose experiment group was higher than that of control groups obviously ( P <0.01).The P-gp expression of every experiment groups rose following the high-dose chemotherapy time.The P-gp expression was higher than the following chemotherapy dose of every group.Conclusion:There were higher P-gp expression in hematopoietic cells with MDR1 gene transplanted into the body of the bone in the Balb/C mice with hepatocarcinoma.

关键词

基因治疗多药耐药阿霉素P-糖蛋白骨髓移植

Keywords

gene therapymultidrug resistanceDoxorubicinP-gpMarrow transplant

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