ZHOU Qin, YAO Zhenwei. The effect of sperm washing on hepatitis B virus concentration in semen[J]. Journal of Chongqing Medical University, 2003,(2):189-191.
ZHOU Qin, YAO Zhenwei. The effect of sperm washing on hepatitis B virus concentration in semen[J]. Journal of Chongqing Medical University, 2003,(2):189-191. DOI: 10.13406/j.cnki.cyxb.2003.02.022.
Objective: To detect hepatitis B virus (HBV) in semen and to find if sperm washing removes HBV DNA. Methods: Amplification by real-time fluorescent quantitative polymerase chain reaction (FQ-PCR) was used to detect viral DNA in semen samples of 57 HBsAg-seropositive patients.Twenty-one semen samples were washed
and spermatozoa were separated by discontinuous Percoll gradient centrifugation and the `swim-up’ method.The HBV DNA in post-washing samples was measured by FQ-PCR. Results: HBV DNA was detected in 15 of 62 semen samples
the positive rate was 24.19%.There were 7 positive cases in 21 samples before washing
the 6 cases except one were positive after washing. Conclusion: Part of semen of HBV infected patients is infective
and sperm washing does not remove HBV.It is cautious to use semen of the HBV infected patients in IVI or IVF.