Objective: To evaluate the detection of ureaplasma urealyticum(UU) with PCR-microplate hybridization (PCR-MPH) and culture

and analyze drug resistant activities. Methods: 496 samples were cultured and drug resistant activities analyzed by Bio-Merieux Mycoplasma-IST

and 212 samples of them were determined by PCR-MPH. Results: The positive rates of PCR-MPH and culture were determined to be 90/212(42.5%) and 78/212(36.8%)

respectively.The specificity and sensitivity of PCR-MPH were 88.1% and 99.4%

respectively.The positive rates of females and males with culture were 101/226(44.7%) and 64/270(23.7%)

respectively.There was significant difference between them(χ 2=24.41

P <0.01).The susceptibility rates of PRI

DOX

JOS

TET

ERY and OFL were 99.4%

91.5%

89.7%

80.0%

49.7% and 25.5%

respectively. Conclusion: PCR-MPH is rapid

sensitive

specific

and is of value in clinical diagnosis and therapy.Higher positive rate of the infected females should be paid attention to.It is very important to select antibiotics correctly for infections of the patients according to results of UU susceptibility tests.