Objective:To study the characteristics of pubertal rat models with insulin resistance and type 2 diabetes mellitus. Methods:Euglycemic-hyperinsulinemic clamp technique was performed in evaluation of insulin sensitivity of two models of pubertal rats

and the correlation between androgen of pubertal rat models and insulin sensitivity was investigated. Results: The plasma testosterone level was decreased and its concentration was positively correlated with insulin sensitivity in male rats.Islet was not obviously impaired by low-dose streptozotocin(STZ) injection (30mg/kg).But type 2 diabetes was successfully established in pubertal Wistar rats by high-fat feeding plus STZ injection .Both plasma triglycerides (TG) and plasma low-density lipoprotein (LDL-c) were increased in pubertal male rats with insulin resistance

and dyslipidemia was aggravated in adult rats with insulin resistance. Conclusion: Our findings suggested that abnormal plasma testosterone level might play an important role in the pubertal rat by high-fat feeding with the occurrence of insulin resistance and type 2 diabetes mellitus.