树突状细胞体外激活的CTL杀伤效应

李用国; 梁增伟; 蔡大川; 兰英华; 周卫平; 任红

doi:10.13406/j.cnki.cyxb.2003.02.005

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树突状细胞体外激活的CTL杀伤效应

更新时间：2024-01-17

- 树突状细胞体外激活的CTL杀伤效应
- CTL killing activity of tumor-specific cytotoxic T lymphocyte activated by dendritic cell
- 重庆医科大学学报 2003年第2期页码：138-139
- 作者机构：
  
  1. 重庆医科大学病毒性肝炎研究所
  2. 重庆医科大学病毒性肝炎研究所,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2003.02.005
  中图分类号： R392.1
- 纸质出版日期：2003，
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[1]李用国,梁增伟,蔡大川,兰英华,周卫平,任红.树突状细胞体外激活的CTL杀伤效应[J].重庆医科大学学报,2003(02):138-139+165.

LI Yongguo, et al. CTL killing activity of tumor-specific cytotoxic T lymphocyte activated by dendritic cell[J]. Journal of Chongqing Medical University, 2003,(2):138-139.
[1]李用国,梁增伟,蔡大川,兰英华,周卫平,任红.树突状细胞体外激活的CTL杀伤效应[J].重庆医科大学学报,2003(02):138-139+165. DOI： 10.13406/j.cnki.cyxb.2003.02.005.

LI Yongguo, et al. CTL killing activity of tumor-specific cytotoxic T lymphocyte activated by dendritic cell[J]. Journal of Chongqing Medical University, 2003,(2):138-139. DOI： 10.13406/j.cnki.cyxb.2003.02.005.

摘要

目的 :研究树突状细胞（dendriticcell

DC）激活的肿瘤特异性细胞毒性T淋巴细胞（CTL）的特异性杀伤效应。方法 :从人外周血中分离出外周血单个核细胞（peripheralbloodmononuclearcell

PBMC）

在GM -CSF和IL - 4的作用下培养 7天诱导出DC

然后PBMC或DC为刺激细胞在体外诱导出HBsAg特异性CTL ;在调节好一定浓度靶细胞（人肝癌细胞系HepG2及人肺腺癌细胞系A5 4 9）中加入效应细胞（DC或PBMC）

4 8h后瑞氏染色计数残存细胞数

计算杀伤率。结果 :DC激活的CTL对HepG2 杀伤率为 71.6 %

而PBMC（APC

非T细胞）激活的CTL杀伤率为 4 3.8%

两者有显著性差异

P <0 .0 1。此外

DC诱导的肝癌细胞抗原特异性CTL对肺癌细胞的杀伤率仅为 2 3%。结论 :DC诱导的CTL比PBMC高效

而且具有较高的特异性。

Abstract

Objective:To investigate CTL killing activity of tumor-specific cytotoxic T lymphocyte activated by dendritic cell. Methods:Isolated peripheral blood mononuclear cells (PBMC)from human peripheral blood and cultured 7days in medium with GM-CSF and IL-4 to get DC.Induced HBsAg-specific CTL by DC or PBMC.Added CTL into some concentration HepG2 or A549.48 hours later

counted survival cells after Wright′s staining to get killing rate.Results: The killing rate of CTL activated by DC to HepG2 was 71.6%;however

that of PBMC was 43.8%.There was significant difference between the two groups( P <0.01).Moreover

the killing rate of HBsAg-specific CTL activated by DC to A549 was only 23%. Conclusion: The killing ability of HBsAg-specific CTL activated by DC was stronger than that of PBMC and it was specific.

关键词

树突状细胞外周血单个核细胞CTL杀伤效应

Keywords

dendritic cellperipheral blood mononuclear cellCTL killing activity

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