细胞凋亡及凋亡相关基因在下肢静脉曲张中的表达及意义

乔正荣; 时德

doi:10.13406/j.cnki.cyxb.2002.01.002

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细胞凋亡及凋亡相关基因在下肢静脉曲张中的表达及意义

更新时间：2024-01-17

- 细胞凋亡及凋亡相关基因在下肢静脉曲张中的表达及意义
- The expression and significance of cell apoptosis and apoptosis genes of varicosity in the lower extremities
- 重庆医科大学学报 2002年第1期页码：5-8
- 作者机构：
  
  重庆医科大学临床学院血管外科,重庆,400016
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2002.01.002
  中图分类号： R654.4
- 纸质出版日期：2002，
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[1]乔正荣,时德.细胞凋亡及凋亡相关基因在下肢静脉曲张中的表达及意义[J].重庆医科大学学报,2002(01):5-8+11.

The expression and significance of cell apoptosis and apoptosis genes of varicosity in the lower extremities[J]. Journal of Chongqing Medical University, 2002,(1):5-8.
[1]乔正荣,时德.细胞凋亡及凋亡相关基因在下肢静脉曲张中的表达及意义[J].重庆医科大学学报,2002(01):5-8+11. DOI： 10.13406/j.cnki.cyxb.2002.01.002.

The expression and significance of cell apoptosis and apoptosis genes of varicosity in the lower extremities[J]. Journal of Chongqing Medical University, 2002,(1):5-8. DOI： 10.13406/j.cnki.cyxb.2002.01.002.

摘要

目的：探讨细胞凋亡和凋亡相关基因在下肢静脉曲张中的表达及意义。方法：采用凋亡细胞原位检测、原位杂交、免疫组化研究８３例曲张静脉和１０例对照组内皮细胞和平滑肌细胞凋亡及相关基因表达。结果：①曲张静脉囊性扩张型Ｅｃｓ及ＳＭＣ凋亡指数显著增高（Ｐ＜０．０１）；②曲张静脉囊性扩张型Ｂｃｌ－２　ｍＲＮＡ阳性细胞表达率均显著低于非囊性扩张型（Ｐ＜０．０５）；③非囊性扩张型其Ｂｃｌ－２基因蛋白阳性细胞表达率均显著高于囊性扩张型和对照组（Ｐ均＜０．０５）；④囊性扩张型其Ｂａｘ　和Ｆａｓ基因蛋白阳性细胞表达率均显著高于非囊性扩张型和对照组（Ｐ均＜０．０５）；⑤Ｂｃｌ－２　ｍＲＮＡ表达阳性细胞数和表达率（０．２５±０．２３）与Ｂｃｌ－２基因蛋白（０．３０±０．２７）呈显著正相关，相关系数ｒ分别为０．７９５３８１（Ｐ＝０．００１９８１）和０．６８８２１６（Ｐ　＝０．０００８４４）。结论：①细胞过度凋亡导致静脉血管重塑，可能是静脉曲张发病机制之一；②曲张静脉囊性扩张型其ＥＣｓ和ＳＭＣ过度凋亡可能与Ｂｃｌ－２　ｍＲＮＡ、Ｂｃｌ－２蛋白低水平表达及Ｂａｘ、Ｆａｓ高水平表达相关。

Abstract

Objective: Exploring the expression and significance of cell apoptosis and apoptosis genes of varico-sity in the lower extremities. Methods:By using TUNEL

hybridization in situ

immunohistochemical method

83 patients with varicosity and 10 controls were studied.Results: Apoptosis index of ECs and SMC in cystic dilata-tions were compared with those of non-cystic dilatations and controls with significant difference (P <0.01). The numbers and index of Bcl-2 mRNA positive ECs and SMC expression were greatly lower in cystic dilatations than those in non-cystic dilatations (P<0.05). Bcl-2 mRNA expression in non-cystic dilatations were markedly higher than that in controls (P<0.05). The numbers and index of protein Bcl-2 positive Ecs and SMC were significantly increased in non-cystic dilatations compared with those of cystic dilatations and control (P <0.05). The numbers and index of protein Bax and Fas positive expression increased markedly in cystic dilatations compared with those of non-cystic dilatations and control (P<0.05). Expressions of Bcl-2 mRNA were positively correlated with that of protein Bcl-2 (r=0.795381

P=0.001981). Conclusion: Over-apoptosis led to venous remode-ling may be one of the mechanism of varicosity. Over-apoptosis of ECs and SMC in cystic dilatations may correlate with low level expression of Bcl-2 mRNA and protein

high level expression of Bax

Fas and ratio of Bcl-2/Bax.

关键词

静脉曲张细胞凋亡凋亡基因

Keywords

Varicositycell apoptosisApoptosis genes

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