用Wistar大鼠门静脉部分结扎制作门脉高压动物模型

王继见; 高根五; 刘长安; 姚榛祥

doi:10.13406/j.cnki.cyxb.2001.03.025

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用Wistar大鼠门静脉部分结扎制作门脉高压动物模型

更新时间：2024-01-17

- 用Wistar大鼠门静脉部分结扎制作门脉高压动物模型
- Preparation of portal hypertensive animal model bypartial portal-vein ligation in Wistar rats
- 重庆医科大学学报 2001年第3期页码：302-304
- 作者机构：
  
  1. 重庆医科大学第二临床学院普外科
  2. 重庆医科大学临床学院普外科,重庆,400010
  3. ,重庆,400016
- 作者简介：
- 基金信息：
  
  重庆市卫生局科学基金支助（97－09）
- DOI：10.13406/j.cnki.cyxb.2001.03.025
  中图分类号： R-332
- 纸质出版日期：2001，
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[1]王继见,高根五,刘长安,姚榛祥.用Wistar大鼠门静脉部分结扎制作门脉高压动物模型[J].重庆医科大学学报,2001(03):302-304.

WANG Ji-jian, et al. Preparation of portal hypertensive animal model bypartial portal-vein ligation in Wistar rats[J]. Journal of Chongqing Medical University, 2001,(3):302-304.
[1]王继见,高根五,刘长安,姚榛祥.用Wistar大鼠门静脉部分结扎制作门脉高压动物模型[J].重庆医科大学学报,2001(03):302-304. DOI： 10.13406/j.cnki.cyxb.2001.03.025.

WANG Ji-jian, et al. Preparation of portal hypertensive animal model bypartial portal-vein ligation in Wistar rats[J]. Journal of Chongqing Medical University, 2001,(3):302-304. DOI： 10.13406/j.cnki.cyxb.2001.03.025.

摘要

目的：用Ｗｉｓｔａｒ大鼠作门静脉部分结扎制作门脉高压动物模型，探索适宜的制模条件。方法：用各种型号钝头直针作门静脉部分结扎。结果：假手术组，用１８号、２０号钝头直针组，直到术后第５天仍无门脉高压形成。用１４号、１６号钝头直针组，术后门脉压力均升高，但１４号钝头直针组大鼠术后表现明显拒食、精神萎靡、体重减轻、腹水形成并相继死亡。１６号钝头直针组，术后０．５小时门脉压力即显著升高，７２小时形成稳定的门脉高压。结论：Ｗｉｓｔａｒ大鼠门静脉部分结扎致门脉高压动物模型，１６号钝头直针外径大小最合适，制模既能成功，又无动物死亡，术后各方面情况均与假手术动物相似。

Abstract

Objective:Portal hypertensive animal model was prepa red by partial portal-vein ligation in Wistar rats. Adaptive condition of preparation of animal model was researched. Methods:Partial portal-vein li gation was made with various types of blunt-tip needles. Results:P ortal hypertension had not yielded on the fifth post-operation day in sham-operation

gauge 18 and gauge 20 groups.Portal pressure had increased in the two groups of gauge 14 and gauge 16. In the gauge 14 group

the rats had expressed anepithymia

loss of body weight

ascites formation and died one by one.Portal pressure had increased significantly in the gauge 16 group in 0.5 post-operative hour and formed stable portal hypertension in 72 post-operative hour.Conclusion:The size of gauge 16 blund-tip needle fits well in the preparation of portal hypertensive animal model in Wistar rats by partial portal-vein ligation.The preparation of the model is successful and all rats are alive as well.The post-operative condition is similar to that of sham-operation.

关键词

门静脉高压门静脉部分结扎Wistar大鼠

Keywords

Portal hypertensionPartial portal-vein ligationWistar rats

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