碱性成纤维细胞生长因子抗局灶性脑缺血/再灌注引发神经细胞凋亡作用的实验研究

杨琴; 胡常林

doi:10.13406/j.cnki.cyxb.2000.04.004

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碱性成纤维细胞生长因子抗局灶性脑缺血/再灌注引发神经细胞凋亡作用的实验研究

更新时间：2024-01-17

- 碱性成纤维细胞生长因子抗局灶性脑缺血/再灌注引发神经细胞凋亡作用的实验研究
- Experiment study of basic fibroblast growth factor reducing neuronic apoptosis following cerebral ischemia/reperfusion in rat
- 重庆医科大学学报 2000年第4期页码：345-347
- 作者机构：
  
  1. 重庆医科大学临床学院神经内科,重庆,400016
  2. 重庆医科大学第二临床学院神经内科,重庆,400010
  3. 重庆医科大学临床学院神经内科!重庆　400016重庆医科大学第二临床学院神经内科,重庆,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2000.04.004
  中图分类号： R743.31
- 纸质出版日期：2000，
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[1]杨琴,胡常林.碱性成纤维细胞生长因子抗局灶性脑缺血/再灌注引发神经细胞凋亡作用的实验研究[J].重庆医科大学学报,2000(04):345-347.

Experiment study of basic fibroblast growth factor reducing neuronic apoptosis following cerebral ischemia/reperfusion in rat[J]. Journal of Chongqing Medical University, 2000,(4):345-347.
[1]杨琴,胡常林.碱性成纤维细胞生长因子抗局灶性脑缺血/再灌注引发神经细胞凋亡作用的实验研究[J].重庆医科大学学报,2000(04):345-347. DOI： 10.13406/j.cnki.cyxb.2000.04.004.

Experiment study of basic fibroblast growth factor reducing neuronic apoptosis following cerebral ischemia/reperfusion in rat[J]. Journal of Chongqing Medical University, 2000,(4):345-347. DOI： 10.13406/j.cnki.cyxb.2000.04.004.

摘要

目的　观察碱性成纤维细胞生长因子（bFGF）抗局灶性脑缺血/ 再灌注引发神经元凋亡作用。方法　采用线栓法制备大脑中动脉缺血/ 再灌注模型。术后首次即刻分别腹腔注射bFGF和生理盐水，以后每天一次，连用7天。TUNEL 法原位检测缺血后第1、2、3、5、7天脑石蜡切片含DNA 碎片的凋亡细胞。结果　正常组、假手术组、缺血组对侧半球每张切片有0～8个凋亡细胞，缺血/ 再灌注后缺血区凋亡细胞明显增多，缺血48h达高峰，以后逐渐降低，第7天仍可见凋亡细胞。bFGF组凋亡细胞数显著低于缺血/ 再灌注生理盐水对照组。结论　缺血/ 再灌注后缺血区神经元发生了凋亡，bFGF具有抗凋亡作用。

Abstract

Objective To ascertain the effect of basic fibroblast growth factor(bFGF) reducing neuronic apoptosis. Methods The animal models of the right middle cerebral artery ischemia/reperfusion was established by suture method in adult wistar rats. The bFGF(100μg/kg) in physiological saline

or physiological saline alone

was injected intraperitoneally immediately after cerebral ischemia

then once daily

for 7 days.We measured brain apoptotic cells exhibiting DNA fragmentation in paraffin sections with the method of a terminal deoxynucleotidylltransferase(TdT)- mediated dUTP- fluorescein nick end- labelling (TUNEL) after ischemia 2 hour/reperfusion for the first

second

third

fifth and seventh day. Results There were 0～8 apoptotic cells per section in normal or sham-operated groups. After cerebral ischemia/reperfusion

the apoptotic neuron number increased significantly in the ischemic region. After 48h

the apoptotic nouron decreased gradually

up to the seventh day

there were also apoptotic neuron. The number of apoptotic neurons in the bFGF- treated group differed signifficantly from that in the non-treated control group (Ｐ<0.05). Conclusion This study suggested that ischemia/reperfusion may induce neuronic apoptosis and bFGF shows the effect of reducing neuronic apoptosis.

关键词

缺血/再灌注凋亡碱性成纤维细胞生长因子

Keywords

Ischemia/reperfusionapoptosisBasic fibroblast growth fact?

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