Objective The levels of EGF were observed in human serum and bronchial washing fluid (BWF) from patients with lung cancer. Methods The levels of EGF were measured by radioimmunoassay (RIA) in human serum and bronchial washing fluid (BWF) from patients with lung cancer (LC) and patients with benign pulmonary disease(BPD) and normal controls (NC).The correlation between the levels of BWF EGF and the levels of serum EGF were studied in LC patients .Results The levels of serum EGF and the levels of BWF EGF in patients with LC were significantly higher(P<0.05) than those in patients with BPD and NC The levels of serum EGF and the levels of BWF EGF were not related to the histologic type and clinical stage of lung cancer. The level of BWF EGF had positive correlation with serum level of it in LC patients. (r=0.77

P<0.05) Conclusion The determination of EGF levels in serum and in BWF may be a helpful method for diagnosis of LC.