血清AFP异质体印迹法和γ-GTⅡ定性、定量检测对原发性肝癌诊断价值的探讨

庞春梅; 沈鼎明; 王景

doi:10.13406/j.cnki.cyxb.2000.03.013

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血清AFP异质体印迹法和γ-GTⅡ定性、定量检测对原发性肝癌诊断价值的探讨

更新时间：2024-01-17

- 血清AFP异质体印迹法和γ-GTⅡ定性、定量检测对原发性肝癌诊断价值的探讨
- Evaluation of serum lectin-reactive α-fetoprotein affinity blotting method and the qualitative,quantitative analysis of γ-glutamyl transpeptidase isozyme Ⅱ in the diagnosis of primary hepatic carcinoma
- 重庆医科大学学报 2000年第3期页码：259-261
- 作者机构：
  
  1. 广州市红十字会医院消化内科
  2. 重庆医科大学第二临床学院消化内科,510220
  3. ,400010
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.2000.03.013
  中图分类号： R735.7
- 纸质出版日期：2000，
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[1]庞春梅,沈鼎明,王景.血清AFP异质体印迹法和γ-GTⅡ定性、定量检测对原发性肝癌诊断价值的探讨[J].重庆医科大学学报,2000(03):259-261+264.

Evaluation of serum lectin-reactive α-fetoprotein affinity blotting method and the qualitative,quantitative analysis of γ-glutamyl transpeptidase isozyme Ⅱ in the diagnosis of primary hepatic carcinoma[J]. Journal of Chongqing Medical University, 2000,(3):259-261.
[1]庞春梅,沈鼎明,王景.血清AFP异质体印迹法和γ-GTⅡ定性、定量检测对原发性肝癌诊断价值的探讨[J].重庆医科大学学报,2000(03):259-261+264. DOI： 10.13406/j.cnki.cyxb.2000.03.013.

Evaluation of serum lectin-reactive α-fetoprotein affinity blotting method and the qualitative,quantitative analysis of γ-glutamyl transpeptidase isozyme Ⅱ in the diagnosis of primary hepatic carcinoma[J]. Journal of Chongqing Medical University, 2000,(3):259-261. DOI： 10.13406/j.cnki.cyxb.2000.03.013.

摘要

目的探讨新的方法检测ＡＦＰ异质体（ＡＦＰ－Ｌ３）和γ－ＧＴⅡ对原发性肝癌（ＰＨＣ）的诊断价值。方法用抗体亲和印迹法检测ＡＦＰ－Ｌ３，并结合γ－ＧＴⅡ定性、定量检测了１１５例各种肝病及消化道肿瘤患者血清。结果　γ-ＧＴⅡ定量检测的敏感性和特异性最高，其次为γ－ＧＴⅡ定性及ＡＦＰ－Ｌ３，联合检测可以提高ＰＨＣ，尤其是小肝癌及低浓度ＡＦＰ肝癌的检出率。结论ＡＦＰ－Ｌ３、γ－ＧＴⅡ是诊断ＰＨＣ敏感性和特异性较高的指标，对小肝癌及低浓度ＡＦＰ肝癌具有重要意义，联合检测可以提高ＰＨＣ的检出率，可以弥补ＡＦＰ的不足，有助于ＰＨＣ的早期诊断。

Abstract

Objective To evaluate the effect of Lectin-reactive α-fetoprotein(AFP-L3) and γ-GT Ⅱ on the diagnosis of primary hepatic carcinoma(PHC). Methods Lectin-reactive α-fetoprotein(AFP-L3) by means of antibody-affinity blotting method

and γ -GT Ⅱ by qualitative

quantitative analysis were determined in 115 cases

including various liver diseases and gastrointestinal tumors. Results The quantitative analysis of γ-GT Ⅱ had the highest sensitivity and specificity followed by the qualitative analysis of γ -GT Ⅱ and AFP-L3. The combination assay of them could raise the diagnosis positivity of PHC

especially the small PHC and low and negative AFP. Co nclusion AFP-L3 and γ -GTⅡ are two potent tumor markers for PHC. The combination assay of the two markers is valuable in the diagnosis of PHC

especially in detecting PHC patients with small size and negative or low AFP.

关键词

原发性肝癌甲胎蛋白异质体:γ-谷氨酰转肽酶Ⅱ抗体亲和印迹法联合检测

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