The relationship between the common mode of HBV immunological marker in serum and the quantity of HBV- DNA[J]. Journal of Chongqing Medical University, 2000,(2):169-170.
The relationship between the common mode of HBV immunological marker in serum and the quantity of HBV- DNA[J]. Journal of Chongqing Medical University, 2000,(2):169-170. DOI: 10.13406/j.cnki.cyxb.2000.02.022.
Objective:To study the relationship between the common mode of HBV immunological marker in serum and the quantity of HBV-DNA. Methods:150 samples were detected by fluorescence quantitative PCR (FQ-PCR) and ELISA kit respectively. Results:In 21HBsAg+/HBeAg+/HbcAb+samples
the FQ-PCR were all positive with 3.5×10 8copies on average
in 6 HBsAg+/HBeAg+samples
the HBV-DNA were all positive and the mean was 1.2×10 9copies. in 27 HBsAg+/HBeAb+/HBcAb+samples 12 samples were positive tested FQ-PCRwith 7.5×10 6 copies
on average; In 24 HBsAg+/HBcAb+samples
8 samples were positive and the amount was 8.5×10 5
the residual HBsAb+and HBsAg -/HBsAb-/HBeAg-/HBeAb-/HBcAb- samples were negative by FQ-PCR. Conclusion:This indicates the FQ-PCR can be used to detect the true state of HBV infection and its replication. The FQ-PCR is helpful to diagnose the hepatitis B
and to select the therapeutic project and to monitor the medication.
关键词
乙型肝炎病毒标志物模式定量聚合酶链反应荧光光度测量法
Keywords
hepatitis B virusmarkerModeQuantitative PCRFluorphotimetry