The purpose of this work was to study the effect of Guangxi Phyllanthus urinaria on anti-duck hepatitis virus(DHBV) in vivo and provide an experimental basis for clinical treatment of patients with hepatitis B. The Chongqing duck hepatitis B virus model was treated with Phyllanthus urinaria once a day for a month at doses of 1g/kg、 5g/kg、10g/kg of body weight. DHBV DNA in serum was detected four times from the beginning to the end of the treatment

ALT and AST in serum were detected simultaneously. Phyllanthus urinaria could significantly lower the serum DHBV DNA level. After 1 week stopping treatment

serum DHBV DNA concentrations in Phyllanthus urinaria-treated ducks did not increase significamly. The study confirms the safety and potent antihepadnaviral activity of Phyllanthus urinaria in vivo.