甲基斑蟊胺诱导HepG<sub>2</sub>细胞凋亡的实验研究

唐俐; 段积华

doi:10.13406/j.cnki.cyxb.1999.04.003

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甲基斑蟊胺诱导HepG₂细胞凋亡的实验研究

更新时间：2024-01-17

- 甲基斑蟊胺诱导HepG₂细胞凋亡的实验研究
- Apoptosis of human HepG₂ induced by N-methylcantharidinimidum
- 重庆医科大学学报 1999年第4期页码：343-346
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1999.04.003
  中图分类号： R735.7
- 纸质出版日期：1999，
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[1]唐俐,段积华.甲基斑蟊胺诱导HepG_2细胞凋亡的实验研究[J].重庆医科大学学报,1999(04):343-346.

Tang Li, et al. Apoptosis of human HepG₂ induced by N-methylcantharidinimidum[J]. Journal of Chongqing Medical University, 1999,(4):343-346.
[1]唐俐,段积华.甲基斑蟊胺诱导HepG_2细胞凋亡的实验研究[J].重庆医科大学学报,1999(04):343-346. DOI： 10.13406/j.cnki.cyxb.1999.04.003.

Tang Li, et al. Apoptosis of human HepG₂ induced by N-methylcantharidinimidum[J]. Journal of Chongqing Medical University, 1999,(4):343-346. DOI： 10.13406/j.cnki.cyxb.1999.04.003.

摘要

为探讨甲基斑蟊胺对ＨｅｐＧ２细胞凋亡的诱导作用，揭示其抗癌机制，用ＭＴＴ法观察药物对细胞的生长抑制作用，用ＴＵＮＥＬ法、流式细胞仪及光镜等技术研究癌细胞凋亡。结果表明药物对癌细胞有明显的抑制生长作用；光镜可见染色质浓缩、核碎裂等改变；流式细胞仪可检测到凋亡峰；６ｍｇ／ｍｌ药物作用５天，ＴＵＮＥＬ法强阳性。提示甲基斑蟊胺可诱导ＨｅｐＧ２细胞凋亡。

Abstract

To demonstrate the induction of apoptosis of human HepG

（Hepatocellular Carcinoma） cell lineN-methylcantharidinimidum in order to investigate the mechanism of its anticancer action. MTT method

TUNIflow cytometry（FCM） and light microscopy were used. HepG2 cells treated with N-ethylcantharidinimidumunderwent cell proliferation inhibition;typical nuclear condensation and fragmentation can be detected by lightmicroscopy;apoptotic peaks were also detected on FCM

and the TUNEL-positive cells can be seen after beingtreated with N-methylcantharidinimidum at 6mg/ml for 5days. N-methylcantharidinimidum can induce apoptosisof HepG2 cells in a certrain concentration.

关键词

甲基斑蟊胺细胞凋亡肝脏肿瘤细胞株

Keywords

N-methylcantharidinimidumapoptosisHepG2

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甲基斑蟊胺诱导HepG2细胞凋亡的实验研究

Apoptosis of human HepG2 induced by N-methylcantharidinimidum

DOI：10.13406/j.cnki.cyxb.1999.04.003

摘要

Abstract

关键词

Keywords

references

甲基斑蟊胺诱导HepG₂细胞凋亡的实验研究

Apoptosis of human HepG₂ induced by N-methylcantharidinimidum