Establishment and Identification of insulin-resistant cell model induced with insulin[J]. Journal of Chongqing Medical University, 1998,(4):335-337. DOI: 10.13406/j.cnki.cyxb.1998.04.012.
This paper describes the method of establishment of insulin-resistant primary rat hepatocyte mode] and HepG2 human hepatocellular carcinoma cell model induced with high concentration of insulin.It can decrease the insulin-stimulated glucose uptake and metabolism of the rat hepatocytes and HepG2 cells
and caused them to be insulin resistant.The method is simple
practicable and reliable which can be widely used fo~ the studies of insulin resistance