人实体瘤抗癌药物敏感试验MTT法的建立

汤为学; 骆云鹏; 王瑞雪

doi:10.13406/j.cnki.cyxb.1992.02.007

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人实体瘤抗癌药物敏感试验MTT法的建立

更新时间：2024-01-17

- 人实体瘤抗癌药物敏感试验MTT法的建立
- DETERMINATION OF ANTICANCER DRUG SENSITIVITY TEST BY THE MTT COLO RIMETRIC ASSAY FOR HUMAN SOLID TUMORS
- 重庆医科大学学报 1992年第2期页码：103-108
- 作者机构：
  
  重庆医科大学病理生理教研室
- 作者简介：
- 基金信息：
  
  卫生部资助科研课题
- DOI：10.13406/j.cnki.cyxb.1992.02.007
  中图分类号：
- 纸质出版日期：1992，
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[1]汤为学,骆云鹏,王瑞雪.人实体瘤抗癌药物敏感试验MTT法的建立[J].重庆医科大学学报,1992(02):103-108.

DETERMINATION OF ANTICANCER DRUG SENSITIVITY TEST BY THE MTT COLO RIMETRIC ASSAY FOR HUMAN SOLID TUMORS[J]. Journal of Chongqing Medical University, 1992,(2):103-108.
[1]汤为学,骆云鹏,王瑞雪.人实体瘤抗癌药物敏感试验MTT法的建立[J].重庆医科大学学报,1992(02):103-108. DOI： 10.13406/j.cnki.cyxb.1992.02.007.

DETERMINATION OF ANTICANCER DRUG SENSITIVITY TEST BY THE MTT COLO RIMETRIC ASSAY FOR HUMAN SOLID TUMORS[J]. Journal of Chongqing Medical University, 1992,(2):103-108. DOI： 10.13406/j.cnki.cyxb.1992.02.007.

摘要

本文探讨用MTT法进行人实体瘤抗癌药敏试验的条件和影响因素

结果表明该法之最适肿瘤细胞浓度为0.5—1×10

个/孔

最适培养时间为三天.167例实体瘤的八种抗癌药物敏感试验结果说明其与肿瘤细胞生长特性、病理类型和肿瘤标本取材部位等因素有关.MTT法有操作简单、重复性好、成功率较高、便于推广等优点

Abstract

We studied the methodology of 3-(4

5-dimethyl-2-lhiazoly) )2

5-diphenyl-2H-tetrazolium bromide(MTT) colorimetric assay for anticancer drug sensitivity test of human solid tumors. Results showed that the optimal cell concentration per well is 0.5-1 × 10~5

the optimal incubation period for the action of anticancer drugs on tumor cells is three days. The concentration of anticancer drugs used should be varied with different drugs. We observed 167 samples of human solid tumors. The applicability of this test is concerned with the growth characteristics of tumor cells

histological types of malignancy

and the resected portion of the tumor specimen. The MTT colorimetric assay is a simple

rapid and reliable method for anticancer drug sensitivity test of human solid tumors

关键词

MTT法肿瘤抗癌药物敏感试验

Keywords

MTT colorimetr ic assaytumorAnticancer drug sensitivity

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