妊娠特异性β<sub>1</sub>糖蛋白的分离纯化及特异抗血清的制备

吕懿娟; 何赐科; 冯涛; 赵瑜

doi:10.13406/j.cnki.cyxb.1991.02.001

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妊娠特异性β₁糖蛋白的分离纯化及特异抗血清的制备

更新时间：2024-01-17

- 妊娠特异性β₁糖蛋白的分离纯化及特异抗血清的制备
- PURIFI CATION OF PREGNANCY SPECIFIC B₁ GLYCOPROTEIN（SP₁）AND SPECIFIC ANTISERUM PREPARATION
- 重庆医科大学学报 1991年第2期页码：91-96
- 作者机构：
  
  重庆医科大学生化教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1991.02.001
  中图分类号：
- 纸质出版日期：1991，
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[1]吕懿娟,何赐科,冯涛,赵瑜.妊娠特异性β_1糖蛋白的分离纯化及特异抗血清的制备[J].重庆医科大学学报,1991(02):91-96.

Lu Yijuan Department of Biochemistry. PURIFI CATION OF PREGNANCY SPECIFIC B₁ GLYCOPROTEIN（SP₁）AND SPECIFIC ANTISERUM PREPARATION[J]. Journal of Chongqing Medical University, 1991,(2):91-96.
[1]吕懿娟,何赐科,冯涛,赵瑜.妊娠特异性β_1糖蛋白的分离纯化及特异抗血清的制备[J].重庆医科大学学报,1991(02):91-96. DOI： 10.13406/j.cnki.cyxb.1991.02.001.

Lu Yijuan Department of Biochemistry. PURIFI CATION OF PREGNANCY SPECIFIC B₁ GLYCOPROTEIN（SP₁）AND SPECIFIC ANTISERUM PREPARATION[J]. Journal of Chongqing Medical University, 1991,(2):91-96. DOI： 10.13406/j.cnki.cyxb.1991.02.001.

摘要

本文采用硫酸铵盐析、DE-52离子交换层析、Sephadex G-200凝胶过滤及抗SP

免疫亲和柱层析等方法从胎盘后血分离妊娠特异性β

民糖蛋白（SP

）所得SP

纯化约207倍.纯化的SP

与德国Behringwerke AG的SP

抗血清在双向免疫扩散时有沉淀反应;免疫电泳时纯化SP

移动的位置与β蛋白相当.聚丙烯酰胺凝胶电泳时纯化的SP

呈现一条主带其分子量为90

000左右.免疫家免所得的抗血清经血浆蛋白吸收后得特异SP

抗血清在双向免疫扩散时与正常男子或非孕妇女血清均无沉淀反应;与孕血反应形成的沉淀线与德国Behring werke AG的SP

抗血清所形成的沉淀线相连;交义免疫电泳时呈现一个峰.此抗血清已成功地用于建立酶联免疫法测定SP

含量

Abstract

The authers employed combinations of sequential (NH4)2 SO4 precipitation

DE-52 ion exchange chromatography

Sephadex G-200 gel filtration to isolate SP1 from retroplacental blood collected during labour. Anti-SP1 affinity chromatography has proven helpful in purification. The final product was shown about 207 fold of purification. Purified SP1 reacts with anti-SP1 serum from Behringwerke AG

migrates as p globulin in immunoelectrophoresis. SDS polyacrylamide gradient gel electroph-oresis indicates a major band with molecular weight around 90000. The antiserum obtained by immunization of rabbits with purified SP1 after appropriate absorption shows a single peak in cross immunoelectrophoresis

fails to react with serum from normal nonpregnant females and males

yields single line showing identity with SP1 antiserum from Behringwerke AG in immunodiffusion with pregnant plasma. This antiserum has been successfully used in establishing ELISA for SP1

关键词

妊娠特异性β1糖蛋白蛋白质纯化

Keywords

Pregnancy Specific β1 GlycoproteinProtein purification

references

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人canstatin基因的克隆、表达、纯化及其生物活性测定

妊娠特异性β1糖蛋白的分离纯化及特异抗血清的制备

PURIFI CATION OF PREGNANCY SPECIFIC B1 GLYCOPROTEIN（SP1）AND SPECIFIC ANTISERUM PREPARATION

DOI：10.13406/j.cnki.cyxb.1991.02.001

摘要

Abstract

关键词

Keywords

references

妊娠特异性β₁糖蛋白的分离纯化及特异抗血清的制备

PURIFI CATION OF PREGNANCY SPECIFIC B₁ GLYCOPROTEIN（SP₁）AND SPECIFIC ANTISERUM PREPARATION