ELISA法检测红细胞免疫粘附功能的探讨

曹东; 刘国卿; 黄宗干

doi:10.13406/j.cnki.cyxb.1991.01.005

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ELISA法检测红细胞免疫粘附功能的探讨

更新时间：2024-01-17

- ELISA法检测红细胞免疫粘附功能的探讨
- A STUDY ON THE DETECTION OF RED CELL IMMUNE ADHERENCE ACTIVITY WITH ERYTHROCYTES ENZYME-LINKED IMMUNOSORBENT ASSAY
- 重庆医科大学学报 1991年第1期页码：13-15
- 作者机构：
  
  1. 重庆医科大学第一医院血液组
  2. 重庆医科大学检验系临床血液学教研室
  3. 重庆医科大学第一医院血液组重庆医科大学检验系临床血液学教研室
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.1991.01.005
  中图分类号：
- 纸质出版日期：1991，
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[1]曹东,刘国卿,黄宗干.ELISA法检测红细胞免疫粘附功能的探讨[J].重庆医科大学学报,1991(01):13-15+12.

A STUDY ON THE DETECTION OF RED CELL IMMUNE ADHERENCE ACTIVITY WITH ERYTHROCYTES ENZYME-LINKED IMMUNOSORBENT ASSAY[J]. Journal of Chongqing Medical University, 1991,(1):13-15.
[1]曹东,刘国卿,黄宗干.ELISA法检测红细胞免疫粘附功能的探讨[J].重庆医科大学学报,1991(01):13-15+12. DOI： 10.13406/j.cnki.cyxb.1991.01.005.

A STUDY ON THE DETECTION OF RED CELL IMMUNE ADHERENCE ACTIVITY WITH ERYTHROCYTES ENZYME-LINKED IMMUNOSORBENT ASSAY[J]. Journal of Chongqing Medical University, 1991,(1):13-15. DOI： 10.13406/j.cnki.cyxb.1991.01.005.

摘要

红细胞免疫粘附活性（RCIA）是评价红细胞免疫功能的重要指标.作者应用ELISA法首次建立了检测RCIA活性及RBC膜免疫复合物水平的红细胞酶联免疫竞争吸附实验.该方法简便

试剂安全

灵敏度高、重复性好、结果可靠.可望为检测红细胞免疫功能的有效方法之一

Abstract

We established a new method

the E-ELISA

to detect red-cell immune adherence (RCIA) activity and the value of immune complexes on the surface of red cell. This method has a good specificity

reproducibility and easily perform

with a sensitivity of 31.25ng/100ul. Fifty samples from normal human were determined. The RCIA activity was 5445 (3.73 ± 0.22 LgG

ngAHG/10~7RBC and the value of immune complexes on the surface of red cell was 234.7(2.37 + 0.21LgG)ngAHG/10~7RB C. We likely suggest that this method is a good tool for study of immune fanction of erythrocytes

关键词

红细胞红细胞免疫红细胞膜

Keywords

Red CellErythrocvte membraneRed Cell immune

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