Zhou Lan et al.. THE PRODUCTION, CHARACTERIZATION AND PRELIMINARY APPLICATION OF TWO ANTI-VIMENTIN MCABS[J]. Journal of Chongqing Medical University, 1990,(1):1-7.
Zhou Lan et al.. THE PRODUCTION, CHARACTERIZATION AND PRELIMINARY APPLICATION OF TWO ANTI-VIMENTIN MCABS[J]. Journal of Chongqing Medical University, 1990,(1):1-7. DOI: 10.13406/j.cnki.cyxb.1990.01.001.
BALB/C mice were immunized with. Vimeatin. (Vim) isolated from pig lens. Two hybridoma cell liaes (H1C2 and I3B2) continuously secreting anti-Vim McAb (IgG1 subclass)were established by the hybridoma technique.Their chromosome modes are 86 and 90 respectively. The specificity of the two McAbs were tested with immu nob lotting and imrnunohistochemical technique on normal and tumor tissues. The results show that they reacted specifically with Vim in the blot and they are able to stain fibroblasts. fibrocytes. endothelial cells
fibroma
lymphoma. melanoma. etc; but not neuron
liver
pancreas
squamous cell carcinoma of lime
etc
Thein uses in the histoge.netic classification of normal and tumor tissues are briefly discussed