Objective:To construct the recombination adenoviruses Ad-Bcr/Abl-ΔFABD and Ad-Bcr/Abl

and to explore the effect of FABD on the cytoplasm localization and function of Bcr/Abl oncoprotein. Methods:The fragments of bcr/abl and bcr/abl-ΔFABD gene were amplified by PCR and cloned into shuttle vector p Ad Track-CMV. The adenoviruses Ad-Bcr/Abl-ΔFABD and Ad-Bcr/Abl were constructed by Adeasy system and amplified in human embryonic kidney HEK293 cells. The expressions of Bcr/Abl-ΔFABD and Bcr/Abl in 293 T cells were detected by Western blot. The cytoplasm localization of Bcr/Abl-ΔFABD and Bcr/Abl in 293 T cells was detected by indirect immunofluorescence. The effect of Bcr/Abl and Bcr/Abl-ΔFABD on apoptosis and proliferation in 293 T cell was detected by flow cytometry and MTT. Results:The shuttle plasmids of adenovirus were confirmed to be correctly constructed by double digestion and gene sequencing. The recombination adenovirus plasmids was verified by PacⅠ digestion. The expression of GFP indicated the adenoviruses were packed. The constructed adenoviruses were expressed in 293 T cells by Western blot. Bcr/Abl-ΔFABD protein located in the cytoplasm not in nuclear as form of particles and did not depend on the distribution of the F-actin? After treating by IM

the distribution of Bcr/Abl is similar with that of Bcr/Abl-ΔFABD protein. As results of flow cytometry and MTT

effect of Bcr/Abl-ΔFABD on apoptosis in 293 T cell was promoted（P<0.05）and effect on proliferation was reduced（P<0.05）compared with that of Bcr/Abl. Conclusion:The adenoviruses Ad-Bcr/Abl-ΔFABD and Ad-Bcr/Abl are constructed successfully. The distribution of Bcr/Abl-ΔFABD mutation protein has changed. The deletion of FABD inhibit the anti-apoptosis ability and proliferation of Bcr/Abl.