Objective:To seek the differential genes of cancer associated fibroblasts（CAFs）from normal fibroblasts（NFs）that might function in regulating the malignancy of cancer cells of EOC. Methods:CAFs and NFs were isolated from fresh ovarian tissue of patients of epithelial ovarian cancer（EOC）（n=3）and with benign diseases（n=3）. Total RNAs were extracted for detecting the differentially expressed genes between CAFs and NFs through DNA microarray. Gene Ontology and KEGG pathway analysis were conducted using DAVID to analyze the biological function of the deferentially expressed genes. q RT-PCR was applied to verify the reliability of DNA microarray result. Results:Totally 1 636 genes changed were identified in CAFs. q RT-PCR analysis of 6 genes validated the array results. Bioinformatics analysis indicated the likely involvement of these differential genes in carcinogenesis and regulation of actin cytoskeleton

which might promote the malignant behavior of EOC. Conclusion:Differentially expressed genes found in CAFs of EOC provide a basis for further studying the promotive role of CAFs for EOC.