Objective:To compare the bone parameters of mouse calvarial when the two-dimentional image layers or position of analysis region for reconstruction were changed after Micro-CT scanning

under the precondition that the whole calvarial was included by the analysis region. Methods:The calvarial of 4-day-old mice were scanned consecutively by micro-CT. The bone parameters were compared when the layers or position of analysis region for reconstruction were changed under the precondition that the whole calvarial was included by the analysis region. Results:Under the precondition that the whole calvarial was included by the analysis region

increasing or decreasing the layers of analysis region could accordingly lead to the change of bone surface and bone volume of mouse calvarial. And the bone parameters of the layers selected randomly were significantly different from the parameters of the layers recommended by the NRecon of micro-CT. And there maybe difference when the total layers of analysis region was same but only the position was changed. Conclusion:The change of layers of analysis region for reconstruction can influence the result of bone parameters. Selecting the layers of analysis region randomly will affect the bone parameters. The only change of position without the change of layers may have influence on the bone parameters.