Objective:To discuss the role of interleukin-27(IL-27)and its special receptor WSX-1/TCCR in inducing inflammatory effects on human fetal membrane in preterm premature rupture of membrane. Methods:(1)The expression of IL-27 special receptor WSX-1 in fetal membrane of preterm premature rupture of membrane and normal term birth was analyzed by western blot and immunohistochemistry assay.(2)Primary human fetal membrane was isolated and cultured and its survival was identified by RT-PCR.(3)The fetal membranes were divided into control group and the experimental group

treated by 10 ng/m L human recombinant IL-27 and then RNA and protein were extracted. MMP2 and MMP9 m RNA level was detected and their activity was examed by quantitative RT-PCR and Gelatin zymography

respectively. Results:(1)The expression of IL-27 receptor WSX-1 had significant differences between preterm premature rupture of membrane and normal term birth.(2)Primary human fetal membrane was successfully isolated and cultured for processing.(3)Expressions of MMP2 and MMP9 were obviously higher in IL-27 treatment group;P<0.05 was statistically significant. Conclusion:IL-27 could induce human fetal membrane to produce MMP2 and MMP9 in the pathogenesis of preterm premature rupture of membrane.