Objective:To study the expression and clinical significance of hypoxia inducible factor-1α（HIF-1α）and Sema4 D in pancreatic carcinoma and to explore its correlation and clinical significant. Methods:The expression of HIF-1α and Sema4 D in 82 cases of pancreatic cancer and the adjacent tissues were examined by immunohistochemistry（SP）. The differences in the different kinds of tissues were observed

and the relationship between the protein expression and the clinical pathological characteristics combined with the prognosis was analyzed. Results:The positive expressions of HIF-1α and Sema4 D were mainly in the cytoplasm

presenting brownish yellow granules. HIF-1αand Sema4 D expressions in pancreatic cancer tissues were 52.4%（43/82）and 51.2%（42/82）

which were significantly higher than those in precancerous tissues

with statistically significant differences

respectively（P

<

0.05）. The abnormal expression of Hif-1α and Sema4 D was in correlation with the tumor staging（χ

2

=11.398

P=0.000）

lymph node metastasis（χ

2

=20.341

P=0.000）

neural invasion

embolus tumor（χ

2

=23.478

P=0.000）.The level of Sema4 D was significantly associated with HIF-1αexpression（κ=0.438

P=0.000）. HIF-1α（χ

2

=12.098

P=0.001）and Sema4 D（χ

2

=9.644

P =0.002） were the prognostic factors in patients with pancreatic carcinoma. Cox analysis showed that they were not independent prognostic factors of pancreatic cancer. Conclusion:Upregulation of HIF-1 alpha and Sema4 D expression in pancreatic cancer

both of which have significant correlation

both of which associated with Lymph node metastasis of pancreatic cancer

nerve invasion

vascular tumor thrombus

expression of HIF-1 alpha and Sema4 D may suggest that pancreatic cancer patients with late stage and poor prognosis.