Objective:To analyze the vaginal micro-ecological condition and humn papilloma virus（HPV）infection for patients with cervical intraepithelial neoplasia（CIN）before and after loop electrosurgical excision procedure（LEEP）

and to provide references for restoring vaginal micro-ecological condition in the treatment of CIN. Methods:Retrospective analysis has proceed in patients who was diagnosed as CIN with HPV infection and treated by LEEP from January 2015 to June 2016 at the Second Affiliated Hospital of Chongqing Medical University;their vaginal microflora and HPV infection were evaluated before and after LEEP. Results:Totally 383 patients out of 722 patients have with complete follow-up data. Patients with vaginal microflora imbalance accounted for 84.3%（323/383）

and patients with clearly diagnosed pathogens accounted for 38.3%（147/383）;the incidences of bacterial vaginosis（BV）

vulvovvaginal candidiasis（VVC）

trichomonas vaginitis（TV）and aerobe vaginosis（AV）were 27.4%（105/383）

7.0%（27/383）

2.3%（9/383）

1.6%（6/383）

respectively. After LEEP treatment

fifty-one of the 383 patients was positive for HPV persistently

and vaginal microflora imbalance accounted for 76.5%（39/51）for those HPV positive;the incidences of BV and VVC were 35.3%（18/51）and9.8%（5/51）

respectively. The rest of the 332 patients was negative for HPV

vaginal microflora imbalance accounted for 16.3%（45/332）;the incidences of BV and VVC were 6%（20/332）and 1.2%（4/332）

respectively. The incidence of imbalanced vaginal micro-ecological

BV and VVC was significantly higher in patients with HPV positive than in patients with HPV negative post-LEEP（P<0.05）. Conclusion:The imbalanced vaginal micro-ecological is prevalent in CIN patients with HPV infection

and the BV is the most common pathogens. HPV persistent infection for CIN post-LEEP is related to vaginal microflora imbalance and pathogens infection such as BV and VVC

which indicates that is very importent to restore the vaginal micro-ecological balance and clear the pathogens during CIN treatment.