Objective:To establish a stable animal model of intrauterine adhesion by mechanical and infection

to measure the expressions of vascular endothelial growth factor（VEGF） and transforming growth factor-β1（TGF-β1） and to evaluate their relationship with the formation of adhesion

in order to provide a experimental platform for the next research. Methods:The uterus from 6 healthy mature female Chinese pastoral dogs were collected and divided into two groups:the treatment group and the control group. The right uterine endometriums were collected and evaluated using HE staining

Masson’s stain and SP immunohistochemistry. Results:The endometrial thickness of treatment group（2.325±0.399）was thinner than that（3.025±0.466）of control group;the number of endometrial glands of treatment group（4.388±1.774）was significantly fewer than that（16.518±4.421）of control group;the endometrial fibrosis area（0.775 ±0.040） was significantly higher than that（0.329 ±0.062） of control group. The expression levels of VEGF（5.967±0.390）and TGF-β1（6.200±0.306）in endometrium of treatment group were significantly higher than those（1.700±0.823）and（1.100±0.361）in the control group. Conclusion:The animal model of intrauterine adhesions are constructed successfully by mechanical and infective method. The high expressions of VEGF and TGF-β1 may be closely related with the occurrence of intrauterine adhesion.