Objective:To explore the mechanism of Ezhu-containing serum inhibiting the expression of glioma-associated oncogene homolog-1（Gli1

the nuclear transcription factor of hedgehog signaling pathway）and β-catenin（the core factor of wnt signaling pathway）in hepatic stellate cells（HSC）stimulated by leptin. Methods:Thirty sprague-dawley（SD） rats were random Ly divided into 3groups（n=10）

which were treated with Ezhu-decoction

colchicine and physiological saline by gavage for 10 d in order to prepare drug-containing serums. The method of high performance liquid chromatography（HPLC）was used to detect the composition of Ezhu in serum. The HSC during the exponential growth phase were divided into 8 groups:blank control group

model group

hedgehog pathway inhibitors（cyclopamine） group

ezhu group

Ezhu and cyclopamine group

hedgehog pathway agonost（pumorphamine）group

Ezhu and purmorphamine group

colchicine group. HSC were cultured in vitro and stimulated with 100 ng/m L leptin（except for the blank control group）

then were treated separately with the corresponding drug for 24 h. After cells being collected

HSC proliferation was detected using MTT colorimetric assay;the expressions of Gli1 and β-catenin were determined by PT-PCR

Western blot and immunofluorescence

respectively. Results:Compared with those of blank control group

the expressions of Gli1 m RNA and Gli1 proteins and β-catenin m RNA and β-catenin proteins were obviously increased after stimulating the HSC of rats by leptin（All P=0.000）. Compared with those of model group

the expressions of Gli1 m RNA and Gli1 proteins and β-catenin m RNA and β-catenin proteins were decreased obviously（All P=0.000）after being interfered by Hh pathway（cyclopamine）and ezhu-containing serum. Compared with the model group

using ezhu-containing serum to interfere the Hh pathway inhibitors group can make the expression of Gli1 m RNA and Gli1 proteins and β-catenin m RNA and β-catenin proteins decreased obviously（All P=0.000）. Compared with those of the model group

after being interfered by colchicines

the expressions of Gli1 m RNA and Gli1 proteins were decreased obviously（P=0.000

P=0.007）

and β-catenin m RNA andβ-catenin proteins were decreased obviously（All P=0.000）. Compared with those of model group

the expressions of Gli1 m RNA and Gli1 proteins and β-catenin m RNA and β-catenin proteins were increased obviously（All P=0.000）after adding Hh pathway agonist（purmorphamine）. Compared with those of purmorphamine group

the expressions of Gli1 m RNA and Gli1 proteins and β-catenin m RNA and β-catenin proteins decreased obviously（All P=0.000）after using ezhu-containing serum to interfere the purmorphamine group. Conclusion:Ezhu-containing serum plays an important part in inhibition of HSC activation and increment by taking part in hedgehog signaling pathway to regulate the expression of Gli1. Ezhu-containing serum could inhibit the expression of β-catenin（the key factor of Wnt signaling pathway）by regulating the expression of Gli1 in leptin-induced HSC

thus inhibiting liver fibrosis.