Objective:To explore the effect of α-mangostin on proliferation

migration

invasion and tumor angiogenesis of human gastric cancer cell MKN45 and its mechanisms. Methods:MTS was used to study the effect of α-mangostin on proliferation of MKN45 cells. Wound healing and Transwell experiments were used to study the effect of α-mangostin on migration and invasion of MKN45 cells in vitro

respectively. Rat arterial ring experiment was used to examine the effect of α-mangostin on tumor angiogenesis. ELISA was used to examine the effect of α-mangostin on expression of vascular endothelial growth factor（VEGF）

tumor necrosis factor-α（TNF-α）

transforming growth factor-β（TGF-β）and basic fibroblast growth factor（b FGF）to explore the potential mechanisms of α-mangostin on regulation of tumor angiogenesis. Western blot was used to detect the effect of α-mangostin on expression of MMP-2

MMP-9

N-cadherin and E-cadherin to explore the potential mechanisms of α-mangostin on regulation of tumor migration and invasion. Results:The proliferation of MKN45 cells in vitro was significant inhibited by α-mangostin compared with the control group. α-mangostin significantly inhibited the migration and invasion of MKN45 cells

respectively. Rat arterial ring experiment showed that α-mangostin inhibited the tumor angiogenesis induced by tumor cell. The protein expressions of VEGF

TNF-α

TGF-β and b FGF were significant inhibited by α-mangostin. Moreover

the expression of MMP-2

MMP-9 and N-cadherin were significant downregulated while E-cadherin was significant upregulated. Conclusion:α-mangostin has anti-proliferation

anti-migration

anti-invasion and antiangiogenesis effects on MKN45 cell

and the potential mechanisms may due to:（1）inhibits the tumor cell migration and tumor angiogenesis-related proteins expression;（2）inhibits the process of epithelial-mesenchymal transition of MKN45 cells.