Objective:To examine expression patterns of monocarboxylate transporter 1

4 and lactate transporting in U251 malignant glioma cells with oxygen-glucose deprivation（OGD）so as to explore the possible the role of MCT 1

4 proliferating

aggressiveness in human glioma cells. Methods:U251 cells were randomly divided into two groups

namely

control group and OGD group. The cultures were incubated for certain periods（1

3

6 hours）with oxygen-glucose deprivation. Cell survival rate was tested by MTT assay

cell morphology was observed by HE staining. The lactic acid concentration was measured by lactate assay kit. The expression patterns of MCT1

4 were detected by immunofluorescence and Western blot. Results:Compared with those of control group

cell viability decreased（the OD values of control group and OGD 1 hour

3 hours

6 hours were 0.584 ±0.031

0.537 ±0.046

0.441 ±0.079

0.200 ±0.011 respectively）and lactate content increased in OGD group with oxygen-glucose deprivation time（the lactate content of control

OGD 1 h

3 h

6 h were 0.150±0.029

0.707±0.022

1.167±0.024

1.325±0.023 respectively）

and lactic acid content decreased by adding 4-CIN

the inhibitor of MCTs（the lactate content of Control

150 μmol/L

425 μmol/L were 0.249±0.012

0.222±0.016

0.167±0.117）. The MCT1

4 expression increased in the OGD group than in control group and continuously increased with the extension of oxygen-glucose deprivation time. Conclusion :MCT1

4 increases and cell viability decreases in U251 glioma cells with the up-regulation of lactic acid content after OGD. The lactic acid content decreases by adding 4-CIN. The above results suggest that MCT1

4 may be contributed to lactate transport of malignant glioma microenvironment

and regulate its proliferation and survival.