8-氯腺苷调节RNA编辑酶ADAR1对乳腺癌SK-BR-3细胞增殖和迁移的影响

谢凤; 莫小梅; 杨生永; 李梨

doi:10.13406/j.cnki.cyxb.001164

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8-氯腺苷调节RNA编辑酶ADAR1对乳腺癌SK-BR-3细胞增殖和迁移的影响

肿瘤相关研究 | 更新时间：2024-01-17

- 8-氯腺苷调节RNA编辑酶ADAR1对乳腺癌SK-BR-3细胞增殖和迁移的影响
- Effects of 8-chloro-adenosine regulating RNA editing deaminase ADAR1 on proliferation and migration of SK-BR-3 breast cancer cells
- 重庆医科大学学报 2017年42卷第11期页码：1411-1416
- 作者机构：
  
  重庆医科大学分子医学与肿瘤研究中心
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001164
  中图分类号： R737.9
- 纸质出版日期：2017，
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谢凤, 莫小梅, 杨生永, 等. 8-氯腺苷调节RNA编辑酶ADAR1对乳腺癌SK-BR-3细胞增殖和迁移的影响[J]. 重庆医科大学学报, 2017,42(11):1411-1416.

Xie Feng, Mo Xiaomei, Yang Shengyong, et al. Effects of 8-chloro-adenosine regulating RNA editing deaminase ADAR1 on proliferation and migration of SK-BR-3 breast cancer cells[J]. Journal of Chongqing Medical University, 2017,42(11):1411-1416.
谢凤, 莫小梅, 杨生永, 等. 8-氯腺苷调节RNA编辑酶ADAR1对乳腺癌SK-BR-3细胞增殖和迁移的影响[J]. 重庆医科大学学报, 2017,42(11):1411-1416. DOI： 10.13406/j.cnki.cyxb.001164.

Xie Feng, Mo Xiaomei, Yang Shengyong, et al. Effects of 8-chloro-adenosine regulating RNA editing deaminase ADAR1 on proliferation and migration of SK-BR-3 breast cancer cells[J]. Journal of Chongqing Medical University, 2017,42(11):1411-1416. DOI： 10.13406/j.cnki.cyxb.001164.

摘要

目的:研究8-氯腺苷(8-chloro-adenosine

8-Cl-Ado)对乳腺癌SK-BR-3细胞增殖和迁移的影响

以及与RNA编辑酶1(adenosine deaminases acting on RNA 1

ADAR1)表达的相关性。方法:蛋白印迹实验检测乳腺癌组织中ADAR1的蛋白表达量以及8-Cl-Ado(10μmol/L)处理乳腺癌SK-BR-3细胞不同时间ADAR1蛋白表达量的变化;MTT法和形态学观察检测不加药组和8-Cl-Ado(10μmol/L)加药组对SK-BR-3细胞增殖的影响;MTT法和伤口愈合实验检测SK-BR-3细胞过表达ADAR1质粒(空白对照组、空载组、ADAR1-P110组、ADAR1-P150组)对8-Cl-Ado作用SK-BR-3细胞增殖及迁移的影响。结果:乳腺癌癌组织中ADAR1-P110和ADAR1-P150蛋白表达量均明显高于癌旁组织(t=9.871

P=0.000;t=7.169

P=0.000);8-Cl-Ado作用SK-BR-3细胞48 h后ADAR1-P110和ADAR1-P150蛋白表达量均明显降低(t=-8.838

P=0.013;t=-19.866

P=0.003);8-Cl-Ado作用SK-BR-3细胞ADAR1-P110组和ADAR1-P150组48 h后增殖抑制率均明显低于空白组(P均为0.000);8-Cl-Ado作用SK-BR-3细胞ADAR1-P110组和ADAR1-P150组48 h后的迁移率均明显高于空白组(P均为0.000)。结论:8-Cl-Ado能明显抑制SK-BR-3细胞增殖和迁移

其作用机制可能与ADAR1的表达下调有关。

Abstract

Objective:To evaluate the effects of 8-chloro-adenosine（8-Cl-Ado）on proliferation

migration and adenosine deaminases acting on RNA 1（ADAR1）expression of SK-BR-3 breast cancer cells. Methods:The expression of ADAR1 protein in breast cancer tissues and SK-BR-3 cells treated by 8-Cl-Ado（10 μmol/L）at different time were evaluated by Western blot. The effects of 8-ClAdo（10 μmol/L）on proliferation of SK-BR-3 at different time were detected by MTT and morphology observation. The effects of ADAR1 plasmid over-expression on proliferation and cell metastasis in SK-BR-3 cells（the blank group

the vector group

the ADAR1-P110 group

the ADAR1-P150 group）were tested by MTT and wound healing assay. Results:The expressions of ADAR1-P110 and ADAR1-P150 protein in breast cancer tissues were significantly higher than those in adiacent tissues（t=9.871

P=0.000;t=7.169

P=0.000）and decreased obviously in SK-BR-3 cells treated by 8-Cl-Ado after 48 hours（t=-8.838

P=0.013;t=-19.866

P=0.003）. The proliferation inhibition rates of SK-BR-3 cells treated by 8-Cl-Ado after 48 hours in the ADAR1-P110 group and the ADAR1-P150 group were lower than those in the control group（All P=0.000）. Furthermore

the migration rates of SK-BR-3 cells treated by 8-Cl-Ado after 48 h in the ADAR1-P110 group and the ADAR1-P150 group were higher than those in the control group（All P<0.000）. Conclusion:8-Cl-Ado could inhibit the proliferation and migration of SK-BR-3 breast cancer cells by the downregulation of the expression of ADAR1 protein

the mechanism of action may be related to the down-regulation of ADAR1 protein ex pression.

关键词

8-氯腺苷乳腺癌SK-BR-3RNA编辑酶ADAR1

Keywords

8-chloro-adenosinebreast cancerSK-BR-3RNA editing deaminaseADAR1

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