Luo Yingying, Huang Ailong, Hu Jieli. Application of CRISPR/Cas9 technology in hepatitis B virus infection[J]. Journal of Chongqing Medical University, 2017,42(7):905-909.
Luo Yingying, Huang Ailong, Hu Jieli. Application of CRISPR/Cas9 technology in hepatitis B virus infection[J]. Journal of Chongqing Medical University, 2017,42(7):905-909. DOI: 10.13406/j.cnki.cyxb.001111.
The clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas)9 system is an adaptive immune system. The sg RNA guides the Cas9 protein to specifically cut target DNA sequence by recognizing the protospacer-adjacent motif(PAM) sequence in target DNA. CRISPR/Cas9 offers several potential advantages
including the ease of customization
higher targeting efficiency and the ability to facilitate multiplex geno-me editing. Due to these advantages
it has been used in gene editing in several kinds of mammalian cells. In this review
we discuss the mechanism of CRISPR/Cas9
application of CRISPR/Cas9 in hepatitis B virus infection and relevant mechanism.
关键词
CRISPR基因剪辑乙型肝炎病毒共价闭合环状DNA
Keywords
clustered regularly interspaced short palindromic repeatsgene editinghepatitis B viruscccDNA