Objective:To study the role of stomatin-like protein 2(SLP-2)in regulating proliferation and differentiation in humans endometrial stromal cell line T-HESC. Methods:The cultured human endometrial stromal cell line T-HESC was used an in vitro model to test the expression of SLP-2 and decidualization marker molecules insulin like growth factor binding protein 1(IGFBP1)

prolactin(PRL)and Desmin in induced decidualized T-HESC by RT-q PCR

Western blot and Immunofluorescence. Small inte-rference RNA(si RNA)was used to knock down of SLP-2 and study the role of SLP-2 in T-HESC cell proliferation and differentiation under decidualization induction. Results :The expression of decidualization marker IGFBP1

PRL and Desmin was significantly increased under induced decidualization in vitro. SLP-2 expression was stimulated in decidualized T-HESC. Knocking down SLP-2by si RNA in decidualized T-HESC critically decreased T-HESC cell proliferation and downregulated proliferation makers Ed U and CCND3. The expression of IGFBP1 and PRL was also significantly reduced following SLP-2 si RNA transfection in decidulized THESC. Conclusion:SLP-2 is involved in regulating T-HESC cell proliferation and differentiation.