Objective:To further confirm if CGA47-66(chromofungin

CHR)plays an inhibitory role on Candida albicans biofilm formation

and to explore its possible mechanism. Methods:MIC50of CHR on floating state Candida albicans was detected by M27-A3 reference method. BIC50 of CHR on biofilm formation was observed by XTT colorimetric assay;colony counting method and XTT colorimetric assay were employed to validate whether CHR had an inhibitory function on Candida albicans biofilm formation

then the antifungal adhesion function of CHR was tested by colony counting method combined with inverted microscope. The further molecular mechanism

and the expressions of adhesion gene Hwp1 were measured by q RT-PCR. Results:Our study firstly demonstrated that CHR could inhibit biofilm formation in a dose dependent manner;the BIC50 was 80 to 160 μmol/L;the MIC50 value of CHR on floating state Candida albicans was 10 to 20μmol/L. CHR could down regulate the expression of adhesion gene Hwp1. The relative expression fold of CHR group was(0.089 5±0.036 0)

the expression of gene Hwp1 in control group was11.17 times than that in the CHR group

and the difference had statistical significance(t=44.008

P=0.001). Conclusion:CHR has the inhibitory effect on Candida albicans biofilm formation

the mechanism may related to reducing adherent ability of Candida albicans.This study provides theoretical basis for the application of CHR in the field of antifungal materials.