TERT和p-Akt对胰岛β细胞凋亡的影响

杜小宇; 甘霞光; 雷小添; 陈刘; 周丽娜; 陈兵; 吴绮楠

doi:10.13406/j.cnki.cyxb.001025

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TERT和p-Akt对胰岛β细胞凋亡的影响

基础研究 | 更新时间：2024-01-17

- TERT和p-Akt对胰岛β细胞凋亡的影响
- Effect of TERT and p-Akt on the apoptosis of islet β cell
- 重庆医科大学学报 2017年42卷第11期页码：1498-1502
- 作者机构：
  
  1. 重庆市涪陵中心医院体检部
  2. 第三军医大学第一附属医院内分泌科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001025
  中图分类号： R587.1
- 纸质出版日期：2017，
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杜小宇, 甘霞光, 雷小添, 等. TERT和p-Akt对胰岛β细胞凋亡的影响[J]. 重庆医科大学学报, 2017,42(11):1498-1502.

Du Xiaoyu, Gan Xiaguang, Lei Xiaotian, et al. Effect of TERT and p-Akt on the apoptosis of islet β cell[J]. Journal of Chongqing Medical University, 2017,42(11):1498-1502.
杜小宇, 甘霞光, 雷小添, 等. TERT和p-Akt对胰岛β细胞凋亡的影响[J]. 重庆医科大学学报, 2017,42(11):1498-1502. DOI： 10.13406/j.cnki.cyxb.001025.

Du Xiaoyu, Gan Xiaguang, Lei Xiaotian, et al. Effect of TERT and p-Akt on the apoptosis of islet β cell[J]. Journal of Chongqing Medical University, 2017,42(11):1498-1502. DOI： 10.13406/j.cnki.cyxb.001025.

摘要

目的:通过抑制磷酸化丝/苏氨酸激酶(phosphorylation serine/threonine kinase

p-Akt)的表达

观察并探讨p-Akt和端粒酶逆转录酶(telomerase reverse transcriptase

TERT)对高糖高脂干预的胰岛β细胞凋亡的影响。方法:将NIT-1细胞随机分成对照组(正常培养)、Akt抑制组(正常培养+Akt抑制剂SH5)、高糖高脂干预组(高糖高脂培养)、高糖高脂+Akt抑制组(高糖高脂培养+Akt抑制剂SH5)

TUNEL法和流式细胞术检测各组细胞凋亡

Western blot检测各组细胞TERT、Akt和p-Akt蛋白表达水平

ELISA检测各组细胞胰岛素分泌。结果:与正常对照组比较[凋亡率:(2.88±1.25)%;胰岛素分泌:(0.65±0.10);p-Akt:(0.80±0.07);TERT:(1.63±0.12)];高糖高脂干预组胰岛β细胞凋亡率(38.25±8.63)%明显增加

胰岛素分泌(0.40±0.09)下降

p-Akt(0.55±0.05)和TERT(1.03±0.05)表达明显下降

差异均有统计学意义(P<0.05);与高糖高脂干预组[凋亡率:(38.25±8.63)%;胰岛素分泌:(0.40±0.09);p-Akt:(0.55±0.05);TERT:(1.03±0.05)]比较

高糖高脂+Akt抑制组细胞凋亡率(62.00±7.13)%明显增高

胰岛素分泌(0.23±0.10)下降

p-Akt(0.28±0.07)和TERT(0.61±0.07)表达明显下降

差异均有统计学意义(P<0.05)。结论:抑制p-Akt表达可增加高糖高脂诱导的胰岛β细胞凋亡

其机制可能与降低了TERT表达水平有关。

Abstract

Objective:To observe the inhibition of phosphorylation serine/threonine kinase（p-Akt）on expression of TERT and to explore the effect and mechanism of apoptosis on islet β cells in high glucose and lipid intervention. Methods:Islet β cells were randomly divided into normal control group

Akt inhibit group

glucose and fatty acid intervention group

glucose and fatty acid intervention +Akt inhibit group. Cell apoptosis was detected by TUNEL and flow cytometry;the protein expressions of p-Akt and TERT were detected by Western blot;insulin secretion was detected by ELISA. Results:Compared with those of control group(apoptosis rate:（2.88±1.25）%;insulin secretion:（0.65±0.10）;p-Akt:（0.80±0.07）;TERT:（1.63±0.12）

islet β cell apoptosis rate（38.25±8.63）%was significantly increased and insulin secretion（0.40±0.09）was decreased significantly

expression of p-Akt（0.55±0.05）and TERT（1.03±0.05）decreased significantly in glucose and fatty acid intervention group

with statistically significant differences（P<0.05）.Compared with those of glucose and fatty acid intervention group(apoptosis rate:（38.25±8.63）%;insulin secretion:（0.40±0.09）;p-Akt:（0.55±0.05）;TERT:（1.03±0.05）

the apoptosis rate（62.00 ±7.13）% was significantly increased and insulin secretion（0.23±0.10）was decreased significantly

expression of p-Akt（0.28±0.07）and TERT（0.61±0.07）was decreased significantly in glucose and fatty acid intervention+Akt inhibit group

with statistically significant differences（P<0.05）. Conclusion:Inhibition of pAkt expression may increase the apoptosis of islet β cells induced by the glucose and fatty acid intervention

and its mechanism may be reduced expression level of TERT.

关键词

胰岛β细胞凋亡糖尿病p-AKT端粒酶逆转录酶

Keywords

islet β cellapoptosisdiabetesp-AKTtelomerase reverse transcriptase

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