Objective:To observe the expression of lipoprotein lipas(LPL)in human glioma cell lines U251 and the effects of nucleocytoplasmic shuttling on their proliferation and growth. Methods:After being incubated with leptomycin B(LMB)for 12 and 24 hours

the cytoplasmic and nuclear proteins of human glioma cells were islolated and analyzed by Western blot;MTT and flow cytometry were used to investigate the cell proliferation and necrosis. Results:After 5 nmol/L and 10 nmol/L of LMB being added

the cells were incubated for 12 and 24 hours;the expression of LPL in the nucleus was significantly increased in a dose dependent manner. The nucleus LPL dramatically increased when LMB incubating for 24 hours at concentration of 10 nmol/L. MTT showed that with the increase of LMB concentration and the extension of the incubation time

the cells proliferation was obviously inhibited [the A value were(0.82±0.08)

(0.71±0.03)

(0.70 ± 0.04)

(0.69 ± 0.07)

(0.68±0.09)

respectively]. U251 cell apoptosis rate was also significantly increased [(2.81±0.33)

(4.17±0.24)

(5.25±0.31)

(6.80±0.42)

respetcively] based on results of flow cytometry.Conclusion:LPL in human glioma U251 cells in nucleocytoplasmic shuttle is CRM1 dependent and CRM1 inhibitor LMB can significantly increase the expression of LMB in the nucleus. With increasing of LMB concentrations and incubation time

the proliferation and apoptosis of U251 cells are also significantly influenced

revealing that nucleocytoplasmic shuttling may have certain effect on the tumor development.