Objective:To build the mice model of pure psychological stress based on combined stimuli. Methods:Thirty male mice were randomized into 2 groups:psychological stress group(n =15) and control group(n =15). After one-week acclimation

the mice model of psychological stress was subjected to social isolation stress(30 days)

and randomized to predator stress(1/day) or social defeat stress(1/day). Open-field test was performed at 24 h after the last psychological stress. Then

the average contents of corticotropin releasing hormone(CRH)

adrenocorticotropic hormone(ACTH)and cortisol(CORT) detected by ELISA and were compared between two groups. Results:The activity was significantly reduced in open-field test in psychological stress group(P<0.01). The average contents of CRH

ACTH and CORT were significantly higher in psychological stress group than in control group(P<0.01).Conclusion:It is a credible method to establish animal model of pure psychological stress through combined stimuli.