LAMA4在胎盘组织中的差异表达及其在滋养细胞中的功能

王平珍; 单楠; 王懿铭; 漆洪波

doi:10.13406/j.cnki.cyxb.001007

您当前的位置：

首页 >

文章列表页 >

LAMA4在胎盘组织中的差异表达及其在滋养细胞中的功能

生殖系统疾病 | 更新时间：2024-01-17

- LAMA4在胎盘组织中的差异表达及其在滋养细胞中的功能
- Expression and function of LAMA4 in the placentas and trophoblast
- 重庆医科大学学报 2016年41卷第7期页码：694-698
- 作者机构：
  
  1. 重庆医科大学附属第一医院产科
  2. 重庆市肿瘤研究所肿瘤内科
- 作者简介：
- 基金信息：
- DOI：10.13406/j.cnki.cyxb.001007
  中图分类号： R714.244
- 纸质出版日期：2016，
扫描看全文
[1]王平珍,单楠,王懿铭,漆洪波.LAMA4在胎盘组织中的差异表达及其在滋养细胞中的功能[J].重庆医科大学学报,2016,41(07):694-698.

Wang pingzhen, Shan Nan, Wang Yiming, et al. Expression and function of LAMA4 in the placentas and trophoblast[J]. Journal of Chongqing Medical University, 2016,41(7):694-698.
[1]王平珍,单楠,王懿铭,漆洪波.LAMA4在胎盘组织中的差异表达及其在滋养细胞中的功能[J].重庆医科大学学报,2016,41(07):694-698. DOI： 10.13406/j.cnki.cyxb.001007.

Wang pingzhen, Shan Nan, Wang Yiming, et al. Expression and function of LAMA4 in the placentas and trophoblast[J]. Journal of Chongqing Medical University, 2016,41(7):694-698. DOI： 10.13406/j.cnki.cyxb.001007.

摘要

目的:探讨层粘连蛋白α4链（laminin alpha4

LAMA4）在胎盘组织中的差异表达及其对滋养细胞功能的影响

揭示其在子痫前期（preeclampsia

PE）发病机制中的作用。方法:收集22例PE和20例正常产妇的胎盘组织

应用免疫组化方法检测LAMA4蛋白在胎盘组织中的表达。采用RNA干扰技术

将滋养细胞分为正常培养对照组、缺氧/复氧（hypoxia/reoxygenation

H/R）培养组及干扰培养组

采用细胞免疫荧光和Western blot法检测LAMA4蛋白在各组细胞中的表达。采用Transwell小室模型检测各培养组细胞的侵袭功能。结果:（1）子痫前期胎盘组织中LAMA4蛋白的表达水平明显低于对照组（P<0.05）。（2）H/R及干扰处理后滋养细胞中LAMA4蛋白的表达水平明显降低（P<0.05）。（3）下调LAMA4的蛋白表达后滋养细胞的侵袭率明显降低（P<0.05）。结论:LAMA4蛋白表达的下调可抑制滋养细胞的侵袭功能

可能参与子痫前期的发病。

Abstract

Objective:To investigate the expression of the laminin alpha4(LAMA4)gene and its effect on invasion of HTR-8/SVneo cells and its role in the pathogenesis of preeclampsia. Methods:Twenty-two patients with severe pre-eclampsia and 20 normal pregnant women were investigated. The expression of LAMA4 was determined by immunohistochemistry. Using RNA interference technology

the trophoblast cells were divided into the normal control group

hypoxia/reoxygenation(H/R)oxygen culture group and LAMA4 interference group. Expression of lama4 in each group was detected by the immune fluorescence and Western blot. The invasive ability of the HTR-8/SVneo cells was tested by transwell. Results:(1)The expression of LAMA4 in severe pre-eclampsia group was dramatically lower than that of control group.(2)The expression of LAMA4 in HTR-8/SVneo cells was significantly decreased after H/R and si RNA treatments.(3)The invasive ability of HTR-8/SVneo cells was deduced after the deduction of LAMA4 expression. Conclusion:Damage of LAMA4 is probably involved in the mechanism of onset of preeclampsia by regulating invasion of trophoblast.

关键词

层粘连蛋白α4链侵袭子痫前期

Keywords

laminin alpha4invasionpreeclampsia

references

浏览量

下载量

CSCD

文章被引用时，请邮件提醒。

提交

工具集

关联资源

LncRNA MYLK-AS1调节miR-141-3p/STMN1轴对胃癌细胞增殖、凋亡和侵袭的影响

PSME1/2通过抑制CDK15的表达促进子宫内膜癌细胞的增殖和侵袭

胎盘生长因子、可溶性fms样酪氨酸激酶-1及糖基化纤连蛋白在子痫前期预测中的应用价值

炎症评分系统对子痫前期诊断价值的研究

MCU通过LETM1维持线粒体钙稳态调节口腔鳞状细胞癌细胞转移