Objective:To explore the regulatory pathways from human adipose-derived stem cells(HADSCs)differentiate into lymphatic endothelial cells(LECs)by vascular endothelial growth factor C(VEGF-C156s)in the early lymphangiogenesis. Methods:HADSCs were randomly divided into 5 groups:blank control group

VEGF-C inducing group(containing 100 ng/m L VEGF-C)

VEGF-C unite inhibitor "a"group(containing 100 ng/m L VEGF-C+5 μmol/L VEGFR3 phosphorylase inhibitor MAZ51)

VEGF-C unite inhibitor "b"group(containing 100 ng/m L VEGF-C+10 μg/m L Anti-Integrinα9 function blocking antibody Y9A2)

VEGF-C unite inhibitor "a"and "b"group. After 10 d culturing

the relative expression level of LECs markers VEGFR3

Integrinα9

LYVE-1 were detected by Western blot and immunofluorescence. The induced cells with VEGF-C were incubated by anti-Integrinα9 function blocking antibody and MAZ51; quantity of cell migration in all groups were calculated using Transwell. Results:Compared with that in induction group

the expression level of LYVE-1 was modestly weakened with blocking VEGFR3 and Integrinα9 pathways(PWestern=0.000

PIF=0.000)

and there was no statistical difference in LYVE-1 level between the two inhibitors at the concentration above(PWestern=0.164

PIF=0.927).Blocking one of the two pathways

the expression of another had no change(PWestern VEGFR3(BVSD)=0.804

PIF VEGFR3(BVSD)=0.528

PWestern Integrinα9(BVSC)=0.914

PIF Integrinα9(BVSC)=O.593). Compared with that of no inhibitors

the quantity of cell migration suffered a modest decrease with blocking VEGFR3 and Integrinα9(P=0.000)

while the numbers of cell migration was further reduced when blocking VEGFR3 and Integrinα9 at the same time(P=0.000). Conclusion:There are two independent pathways:VEGF-C/VEGFR3 and VEGF-C/Integrinα9 in the differentiation process from stem cells into LECs. VEGFR3 and Integrinα9 play major role in the migration of induced cells.