Objective:To explore the significance of combined detection of cystatin C(CysC)

homocysteine(Hcy)

soluble intercellular adhesion molecule-1(s ICAM-1)in the early detection of diabetic nephropathy(DN). Methods:According to urinary albumin creatinine ratio(UACR)

180 diabetic cases were divided into non-diabetic nephropathy group(NDN) and diabetic nephropathy group(DN)

and 90 normal persons were taken as the control(NC). The levels of Hcy

CysC

s ICAM-1 in the three groups were detected

and their relationship with UACR was observed. The ROC curve was drawn and its sensitivity and specificity was calculated. Results:(1)Hcy

CysC

s ICAM-1 and UACR levels were higher in group DN than in group NC and group NDN

with statistically significant differences(P1=0.000

P2=0.000

P3=0.000

P4=0.000). Hcy and CysC levels were higher in group NDN than in group NC(P1=0.000

P2=0.000

P3=0.000

P4=0.000)

and there was no statistical difference in s ICAM-1 between the two groups(P=0.153).(2)In group DN

the sensitivity rates of Hcy

CysC

s ICAM-1 were 81.11%

56.7%

10% respectively;the specificity rates were 47.7%

77.8%

97.7%

38.89%

97.7%;the area under the ROC curve of each index were 0.74

0.78

0.72(P1=0.000

P2=0.000

P3=0.000);95%CI were0.667-0.816

0.707-0.843

0.640-0.794 respectively. For joint detection

the sensitivity was 88.8%

the area under the ROC curve was 0.813

95% CI was 0.748-0.878.(3)Hcy

CysC

s ICAM-1 was positively correlated with UACR(r were 0.028

0.449

0.164 respectively

P1=0.001

P2=0.000

P3=0.047). Conclusion:Hcy

CysC

s ICAM-1 can be detected at early stage of diabetic nephropathy

and are positively correlated with UACR. Combined detection of the three can improve the early detection rate of DN.