Objective:To identify the specific metabolic changes by performing the metabolomics analyses on the serum of patients suf fered from acute ST-segment elevation myocardial infarction(ASTEMI). Methods:Ultraperformance liquid chromatography/electrospray ionization quadruple time-of-flight mass spectrometry(UPLC-Q-TOF-MS/MS) and multivariate statistical analysis combined with statistical analysis unit were used to analyze the serum of ASTEMI patients group(n=15)in comparison with the healthy control group(n=10). Further chemiluminescence detection of dehydroepiandrosterone sulfate(DHEAS)was carried out to verify some of the metabolomics findings from the ASTEMI patients. Results:UPLC-Q-TOF-MS/MS revealed that ASTEMI patients and healthy control individuals exhibited distinct metabolomics profiles. Principal component analysis(PCA)and partial least squares discriminant analysis(PLS-DA)demonstrated that ASTEMI patients and healthy control groups were resided in different regions. By using multivariate statistical analysis and statistical analysis

at least nine metabolites were found to markedly differ in the serum of ASTEMI group from those in the healthy individuals. Some differences revealed by the metabolomics study

such as the significant reduction of DHEAS in the serum of ASTEMI patients

were further confirmed by independent chemiluminescence measure ments(P=0.000). Conclusion:The unbiased metabolomics analyses reveal that there are significant differences between ASTEMI patients and healthy controls at serum metabolite levels. At last nine metabolites in the serum are found to be associated with ASTEMI. These findings may provide new insights regarding the metabolism alterations in ASTEMI patients.