类肿瘤干细胞标记物及缺氧诱导因子-1α在口腔鳞癌组织中的表达及相关性研究

李立峰; 季平; 王俊

doi:10.13406/j.cnki.cyxb.000965

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类肿瘤干细胞标记物及缺氧诱导因子-1α在口腔鳞癌组织中的表达及相关性研究

基础研究 | 更新时间：2024-01-17

- 类肿瘤干细胞标记物及缺氧诱导因子-1α在口腔鳞癌组织中的表达及相关性研究
- Expression and correlation of cancer stem-like cell markers CD44, ALDH1,NANOG and hypoxia inducible factor-1α in oral squamous cell carcinoma
- 重庆医科大学学报 2016年41卷第7期页码：727-732
- 作者机构：
  
  重庆医科大学附属口腔医院颌面外科口腔疾病与生物医学重庆市重点实验室重庆市高校市级口腔生物医学工程重点实验室
- 作者简介：
- 基金信息：
  
  重庆市卫生局重点项目专项资助项目（编号:2013-1-030）
- DOI：10.13406/j.cnki.cyxb.000965
  中图分类号： R739.85
- 纸质出版日期：2016，
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[1]李立峰,季平,王俊.类肿瘤干细胞标记物及缺氧诱导因子-1α在口腔鳞癌组织中的表达及相关性研究[J].重庆医科大学学报,2016,41(07):727-732.

Li Lifeng, Ji Ping, Wang Jun. Expression and correlation of cancer stem-like cell markers CD44, ALDH1,NANOG and hypoxia inducible factor-1α in oral squamous cell carcinoma[J]. Journal of Chongqing Medical University, 2016,41(7):727-732.
[1]李立峰,季平,王俊.类肿瘤干细胞标记物及缺氧诱导因子-1α在口腔鳞癌组织中的表达及相关性研究[J].重庆医科大学学报,2016,41(07):727-732. DOI： 10.13406/j.cnki.cyxb.000965.

Li Lifeng, Ji Ping, Wang Jun. Expression and correlation of cancer stem-like cell markers CD44, ALDH1,NANOG and hypoxia inducible factor-1α in oral squamous cell carcinoma[J]. Journal of Chongqing Medical University, 2016,41(7):727-732. DOI： 10.13406/j.cnki.cyxb.000965.

摘要

目的:检测类肿瘤干细胞（cancer stem-like cell

CSC）标记物（CD44、ALDH1、NANOG）及缺氧诱导因子-1α（hypoxia inducible factor-1α

HIF-1α）在口腔鳞癌组织中的表达并进行相关性分析。方法:应用免疫组化法检测40例重庆医科大学附属口腔医院颌面外科手术切除的口腔鳞癌组织、10例癌旁组织、10例淋巴结转移的淋巴结组织以及10例正常淋巴结组织中CD44、ALDH1、NANOG及HIF-1α的表达

采用Fisher确切概率法及秩和检验分析这些标记物与HIF-1α之间及其与肿瘤分化程度、分期及淋巴结转移的关系。结果:（1）CD44、NANOG、HIF-1α在口腔鳞癌组织与癌旁组织中的表达差异均存在统计学意义(P

CD44

=0.000;P

NANOG

=0.000;P

HIF-1α

=0.000);（2）HIF-1α与CD44在口腔鳞癌中的表达存在相关性（r

=0.498

P=0.027）;（3）CD44、ALDH1、NANOG及HIF-1α在口腔鳞癌组织中的表达与分化程度、临床分期及淋巴结转移均无明显相关性。结论:CD44、NANOG、HIF-1α在口腔鳞癌组织中呈高表达

其中CD44与HIF-1α的表达具有相关性。联合检测CD44、NANOG、HIF-1α可能对口腔鳞癌早期诊断具有重要意义。

Abstract

Objective:To investigate the expression and correlation of cancer stem-like cell（CSC）markers CD44

ALDH1

NANOG and hypoxia inducible factor-1α（HIF-1α） in oral squamous cell carcinoma. Methods:Expression of CD44

ALDH1

NANOG and HIF-1α in 40 cases of oral squamous cell carcinoma tissues

collected form the Affiliated Stomatological Hospital of Chongqing Medical University

10 cases of metastatic lymph node tissues

10 cases of non cancer tissues and 10 cases of normal lymph node tissues were detected using immunohistochemistry.The correlations of the expression of the makers and HIF-1α as well as their correlations with tumor differentiation

histological type

clinical stage and lymphoid metastasis were analyzed using Fisher exact test and KruskalWallis test. Results:（1）The expressions of CD44

NANOG

HIF-1α were significantly higher than those in non-cancer tissues(P

CD44

=0.000;P

NANOG

=0.000;P

HIF-1α

=0.000).（2）Only expression of CD44 was positively correlated with HIF-1α expression in oral squamous cell carcinoma（r

=0.498

P=0.027）.（3）Expression of CD44

ALDH1

NANOG and HIF-1α was not correlated with tumor differentiation

histological type and lymphoid metastasis. Conclusion:The positive expressions of CD44

NANOG and HIF-1α in oral squamous cell carcinoma are higher than those of non-cancer tissues. Expression of CD44 showed significant correlation with HIF-1α expression.This may suggest that the combined examination of CD44

NANOG and HIF-1α may be significant for the early diagnosis of oral squamous cell carcinoma.

关键词

类肿瘤干细胞标记物缺氧诱导因子-1α口腔鳞癌相关性分析

Keywords

cancer stem-like cell markershypoxia-inducible factor-1αoral squamous cell carcinomacorrelation analysis

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