Objective:To explore the role of thioredoxin-interacting protein(TXNIP)mediated NLR family pyrin domain containing 3(NLRP3)inflammasome activation in the pathogenesis of preeclampsia(PE). Methods:Fifteen cases of early-onset PE and 15 cases of normal maternal placental tissue were collected in the First Affiliated Hospital of Chongqing Medical University from December2013 to June 2014. Western blot was used to detect the expression of TXNIP and NLRP3 in placenta. Human transformed primary extravillous trophoblast cell line(HTR8/SVneo)cells were used to establish the model of PE

which were divided into control group

hypoxia reoxygenation(H/R)group

H/R+Scrambled si RNA(H/R+NC)group and H/R+TXNIP si RNA(H/R+si RNA)group. After the treatment

expressions of TXNIP and NLRP3 were detected by Western blot;Caspase 1 activity was measured by Caspase-1 active detection kit;interaction between TXNIP and NLRP3 was tested by Co-IP;invasion and proliferation were detected by real-time cell analyzer instrument. Results:The expression of TXNIP and NLRP3 in placenta in PE group was obviously higher than that of normal group(P=0.003

P=0.002). In the cell model

H/R could increase the expression of TXNIP and NLRP3 obviously(P=0.000

P=0.001)

and interaction between TXNIP and NLRP3 was improved remarkably. After the inhibition of TXNIP

expression of NLRP3 was significantly reduced(P=0.000)

and ASC level was also declined obviously(P=0.001)

and the invasion and proliferation ability of H/R was decreased(P =0.001

P =0.038). Conclusion:TXNIP mediated NLRP3 inflammatory activation plays an important role in the pathogenesis of PE.